An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The nuclear localization pattern and interaction partners of GTF2IRD1 demonstrate a role in chromatin regulation.

Carmona-Mora P, Widagdo J, Tomasetig F, Canales CP, Cha Y, Lee W, Alshawaf A, Dottori M, Whan RM, Hardeman EC, Palmer SJ

GTF2IRD1 is one of the three members of the GTF2I gene family, clustered on chromosome 7 within a 1.8 Mb region that is prone to duplications and deletions in humans. Hemizygous deletions cause Williams-Beuren syndrome (WBS) and duplications cause WBS duplication syndrome. These copy number variations disturb a variety of developmental systems and neurological functions. Human mapping data and analyses ... [more]

Hum. Genet. Oct. 01, 2015; 134(10);1099-115 [Pubmed: 26275350]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
GTF2IRD1 DCAF6	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Carmona-Mora P (2015)	Low/High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

DCAF6

Gene Ontology Biological Process

Gene Ontology Molecular Function

ligand-dependent nuclear receptor transcription coactivator activity [ISO]

Gene Ontology Cellular Component

GTF2IRD1