BAIT

EIF4E

EG668879, Eif4e-ps, If4e, eIF-4E
eukaryotic translation initiation factor 4E
Mus musculus
PREY

EIF4ENIF1

2610509L04Rik, A930019J01Rik, AA410001, AU021239, Clast4, D11Ertd166e, RP23-191E3.3
eukaryotic translation initiation factor 4E nuclear import factor 1
GO Process (0)
GO Function (2)
GO Component (4)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Phosphorylation of eIF4E Confers Resistance to Cellular Stress and DNA-Damaging Agents through an Interaction with 4E-T: A Rationale for Novel Therapeutic Approaches.

Martinez A, Sese M, Losa JH, Robichaud N, Sonenberg N, Aasen T, Ramon Y Cajal S

Phosphorylation of the eukaryotic translation initiation factor eIF4E is associated with malignant progression and poor cancer prognosis. Accordingly, here we have analyzed the association between eIF4E phosphorylation and cellular resistance to oxidative stress, starvation, and DNA-damaging agents in vitro. Using immortalized and cancer cell lines, retroviral expression of a phosphomimetic (S209D) form of eIF4E, but not phospho-dead (S209A) eIF4E or ... [more]

PLoS ONE Apr. 30, 2015; 10(4);e0123352 [Pubmed: 25923732]

Throughput

  • Low Throughput

Curated By

  • BioGRID