Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A novel interaction between FRMD7 and CASK: evidence for a causal role in idiopathic infantile nystagmus.

Watkins RJ, Patil R, Goult BT, Thomas MG, Gottlob I, Shackleton S

Idiopathic infantile nystagmus (IIN) is a genetically heterogeneous disorder of eye movement that can be caused by mutations in the FRMD7 gene that encodes a FERM domain protein. FRMD7 is expressed in the brain and knock-down studies suggest it plays a role in neurite extension through modulation of the actin cytoskeleton, yet little is known about its precise molecular function ... [more]

Hum. Mol. Genet. May. 15, 2013; 22(10);2105-18 [Pubmed: 23406872]

Throughput

  • Low Throughput

Additional Notes

  • Also validated by affinity capture-western and colocalization

Curated By

  • BioGRID