BAIT
PIK3R1
PI3KA
phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
GO Process (54)
GO Function (24)
GO Component (12)
Gene Ontology Biological Process
- B cell differentiation [ISO]
- NFAT protein import into nucleus [ISO]
- aging [IEP]
- cellular glucose homeostasis [ISO, ISS]
- cellular response to UV [ISO]
- cellular response to fatty acid [IEP]
- cellular response to insulin stimulus [IEP, ISO, ISS]
- extrinsic apoptotic signaling pathway via death domain receptors [ISO]
- glucose metabolic process [IMP]
- growth hormone receptor signaling pathway [ISO]
- insulin receptor signaling pathway [IMP, ISS]
- insulin-like growth factor receptor signaling pathway [IMP, ISO, ISS]
- intrinsic apoptotic signaling pathway in response to DNA damage [ISO]
- negative regulation of apoptotic process [ISO, ISS]
- negative regulation of blood pressure [IMP]
- negative regulation of cell-cell adhesion [IMP]
- negative regulation of cell-matrix adhesion [ISO]
- negative regulation of heart rate [IMP]
- negative regulation of muscle cell apoptotic process [IMP]
- negative regulation of osteoclast differentiation [ISO]
- negative regulation of proteolysis [IMP]
- negative regulation of smooth muscle cell proliferation [IMP]
- phosphatidylinositol 3-kinase signaling [ISO]
- phosphatidylinositol phosphorylation [ISS]
- phosphatidylinositol-3-phosphate biosynthetic process [IDA, TAS]
- positive regulation of RNA splicing [ISO, ISS]
- positive regulation of cell migration [IMP, ISO]
- positive regulation of endoplasmic reticulum unfolded protein response [ISO, ISS]
- positive regulation of gene expression [IMP]
- positive regulation of myoblast differentiation [IMP]
- positive regulation of protein phosphorylation [IMP]
- positive regulation of transcription factor import into nucleus [ISO, ISS]
- positive regulation of transcription from RNA polymerase II promoter [ISO, ISS]
- positive regulation of tumor necrosis factor production [ISO]
- protein phosphorylation [ISO]
- protein stabilization [ISO, ISS]
- regulation of phosphatidylinositol 3-kinase activity [IDA]
- response to amino acid [IEP]
- response to cAMP [IDA]
- response to dexamethasone [IEP]
- response to drug [IEP]
- response to endoplasmic reticulum stress [ISO, ISS]
- response to estradiol [IEP]
- response to ethanol [IEP]
- response to fatty acid [IEP]
- response to fructose [IEP]
- response to glucocorticoid [IDA]
- response to growth factor [IEP]
- response to insulin [IDA]
- response to iron(II) ion [IEP]
- response to nutrient [IEP]
- response to progesterone [IEP]
- response to testosterone [IEP]
- response to yeast [IEP]
Gene Ontology Molecular Function- 1-phosphatidylinositol-3-kinase activity [IDA, TAS]
- 1-phosphatidylinositol-3-kinase regulator activity [IDA]
- ATPase binding [IPI]
- ErbB-3 class receptor binding [ISO, ISS]
- calmodulin binding [IPI]
- estrogen receptor binding [IPI]
- insulin binding [ISO]
- insulin receptor binding [ISO, ISS]
- insulin receptor substrate binding [IPI, ISO, ISS]
- insulin-like growth factor receptor binding [ISO, ISS]
- neurotrophin TRKA receptor binding [ISO]
- phosphatidylinositol 3-kinase binding [IPI]
- phosphatidylinositol 3-kinase regulator activity [ISS]
- phosphoprotein binding [IPI]
- platelet-derived growth factor receptor binding [IPI]
- protein C-terminus binding [IDA]
- protein binding [IPI]
- protein domain specific binding [IPI]
- protein kinase binding [IPI]
- protein phosphatase binding [ISO]
- receptor binding [IPI]
- receptor tyrosine kinase binding [IPI]
- transcription factor binding [ISO]
- ubiquitin protein ligase binding [IPI]
- 1-phosphatidylinositol-3-kinase activity [IDA, TAS]
- 1-phosphatidylinositol-3-kinase regulator activity [IDA]
- ATPase binding [IPI]
- ErbB-3 class receptor binding [ISO, ISS]
- calmodulin binding [IPI]
- estrogen receptor binding [IPI]
- insulin binding [ISO]
- insulin receptor binding [ISO, ISS]
- insulin receptor substrate binding [IPI, ISO, ISS]
- insulin-like growth factor receptor binding [ISO, ISS]
- neurotrophin TRKA receptor binding [ISO]
- phosphatidylinositol 3-kinase binding [IPI]
- phosphatidylinositol 3-kinase regulator activity [ISS]
- phosphoprotein binding [IPI]
- platelet-derived growth factor receptor binding [IPI]
- protein C-terminus binding [IDA]
- protein binding [IPI]
- protein domain specific binding [IPI]
- protein kinase binding [IPI]
- protein phosphatase binding [ISO]
- receptor binding [IPI]
- receptor tyrosine kinase binding [IPI]
- transcription factor binding [ISO]
- ubiquitin protein ligase binding [IPI]
Gene Ontology Cellular Component
Rattus norvegicus
PREY
IRS1
HIRS-1
insulin receptor substrate 1
GO Process (25)
GO Function (7)
GO Component (7)
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- JAK-STAT cascade involved in growth hormone signaling pathway [TAS]
- cellular response to insulin stimulus [IMP]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- glucose homeostasis [TAS]
- innate immune response [TAS]
- insulin receptor signaling pathway [IDA, IMP, IPI, TAS]
- insulin-like growth factor receptor signaling pathway [IPI]
- negative regulation of insulin receptor signaling pathway [ISS]
- negative regulation of insulin secretion [IDA]
- neurotrophin TRK receptor signaling pathway [TAS]
- phosphatidylinositol 3-kinase signaling [IDA]
- phosphatidylinositol-mediated signaling [TAS]
- positive regulation of cell proliferation [NAS]
- positive regulation of fatty acid beta-oxidation [IMP]
- positive regulation of glucose import [IMP]
- positive regulation of glucose import in response to insulin stimulus [IDA]
- positive regulation of glucose metabolic process [IMP]
- positive regulation of glycogen biosynthetic process [IMP, NAS]
- positive regulation of insulin receptor signaling pathway [ISS]
- positive regulation of phosphatidylinositol 3-kinase activity [ISS]
- response to insulin [IDA]
- response to peptide hormone [ISS]
- signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Fatty acylated caveolin-2 is a substrate of insulin receptor tyrosine kinase for insulin receptor substrate-1-directed signaling activation.
Here, we demonstrate that insulin receptor (IR) tyrosine kinase catalyzes Tyr-19 and Tyr-27 phosphorylation of caveolin-2 (cav-2), leading to stimulation of signaling proteins downstream of IR, and that the catalysis is dependent on fatty acylation status of cav-2, promoting its interaction with IR. Cav-2 is myristoylated at Gly-2 and palmitoylated at Cys-109, Cys-122, and Cys-145. The fatty acylation deficient mutants ... [more]
Biochim. Biophys. Acta May. 01, 2015; 1853(5);1022-34 [Pubmed: 25667086]
Throughput
- Low Throughput
Curated By
- BioGRID