BAIT

VPS52

ARE1, SAC2, SACM2L, dJ1033B10.5, DADB-91M20.1
vacuolar protein sorting 52 homolog (S. cerevisiae)
GO Process (0)
GO Function (2)
GO Component (2)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

MAGEL2

NDNL1, PWLS, nM15
MAGE-like 2
UPS Project
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The Prader-Willi syndrome proteins MAGEL2 and necdin regulate leptin receptor cell surface abundance through ubiquitination pathways.

Wijesuriya TM, De Ceuninck L, Masschaele D, Sanderson MR, Carias KV, Tavernier J, Wevrick R

In Prader-Willi syndrome (PWS), obesity is caused by the disruption of appetite-controlling pathways in the brain. Two PWS candidate genes encode MAGEL2 and necdin, related melanoma antigen proteins that assemble into ubiquitination complexes. Mice lacking Magel2 are obese and lack leptin sensitivity in hypothalamic pro-opiomelanocortin neurons, suggesting dysregulation of leptin receptor (LepR) activity. Hypothalamus from Magel2-null mice had less LepR ... [more]

Hum. Mol. Genet. Nov. 01, 2017; 26(21);4215-4230 [Pubmed: 28973533]

Throughput

  • Low Throughput

Additional Notes

  • interaction assayed by mammalian protein-protein interaction trap (MAPPIT)

Curated By

  • BioGRID