RAPGEF1
Gene Ontology Biological Process
- Rap protein signal transduction [IMP]
- activation of MAPKK activity [TAS]
- cellular response to cAMP [IDA]
- cellular response to nerve growth factor stimulus [IDA]
- establishment of endothelial barrier [IMP]
- nerve growth factor signaling pathway [IDA]
- neurotrophin TRK receptor signaling pathway [TAS]
- positive regulation of Rap GTPase activity [IDA, IMP]
- positive regulation of neuron projection development [IMP]
- regulation of cell junction assembly [IMP]
- signal transduction [NAS]
- transmembrane receptor protein tyrosine kinase signaling pathway [TAS]
Gene Ontology Molecular Function
RAP1A
Gene Ontology Biological Process
- Rap protein signal transduction [IMP]
- activation of MAPKK activity [TAS]
- blood coagulation [TAS]
- cellular response to cAMP [IDA]
- cellular response to nerve growth factor stimulus [ISS]
- energy reserve metabolic process [TAS]
- establishment of endothelial barrier [IMP]
- nerve growth factor signaling pathway [ISS]
- neurotrophin TRK receptor signaling pathway [TAS]
- platelet activation [TAS]
- positive regulation of ERK1 and ERK2 cascade [ISS]
- positive regulation of Rap GTPase activity [ISS]
- positive regulation of neuron projection development [ISS]
- positive regulation of protein kinase activity [ISS]
- positive regulation of vasculogenesis [ISS]
- protein transport [IDA]
- regulation of cell junction assembly [IMP]
- regulation of insulin secretion [TAS]
- signal transduction [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Biochemical characterization of C3G: an exchange factor that discriminates between Rap1 and Rap2 and is not inhibited by Rap1A(S17N).
A catalytically active fragment of the Rap-specific guanine-nucleotide exchange factor C3G was expressed in E coli. It was purified and its interaction with GTP-binding proteins was investigated using fluorescence spectroscopy. C3G stimulates GDP dissociation from Rap1, but not from Rap2, neither from Bud1, which is believed to be the yeast homologue of Rap1 nor from all other proteins of the ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID