MED7
Gene Ontology Biological Process
Gene Ontology Cellular Component
SRB2
Gene Ontology Biological Process
- RNA polymerase II transcriptional preinitiation complex assembly [IDA]
- negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to chemical stimulus [IMP]
- negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to nutrient levels [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
Gene Ontology Molecular Function- RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly [IDA]
- RNA polymerase II transcription factor recruiting transcription factor activity [IMP]
- TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA]
- core RNA polymerase II binding transcription factor activity [IDA, IGI]
- protein domain specific binding [IGI]
- RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly [IDA]
- RNA polymerase II transcription factor recruiting transcription factor activity [IMP]
- TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA]
- core RNA polymerase II binding transcription factor activity [IDA, IGI]
- protein domain specific binding [IGI]
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
The Nuclear Pore-Associated TREX-2 Complex Employs Mediator to Regulate Gene Expression.
Nuclear pore complexes (NPCs) influence gene expression besides their established function in nuclear transport. The TREX-2 complex localizes to the NPC basket and affects gene-NPC interactions, transcription, and mRNA export. How TREX-2 regulates the gene expression machinery is unknown. Here, we show that TREX-2 interacts with the Mediator complex, an essential regulator of RNA Polymerase (Pol) II. Structural and biochemical ... [more]
Throughput
- Low Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
MED7 SRB2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
MED7 SRB2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
MED7 SRB2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
SRB2 MED7 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 6 | BioGRID | 3618706 | |
MED7 SRB2 | Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps. | Low | - | BioGRID | - | |
MED7 SRB2 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.3189 | BioGRID | 2014821 | |
MED7 SRB2 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | High | - | BioGRID | 615304 |
Curated By
- BioGRID