Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Oxidation of DJ-1-dependent cell transformation through direct binding of DJ-1 to PTEN.

Kim YC, Kitaura H, Taira T, Iguchi-Ariga SM, Ariga H

DJ-1 is an oncogene and also a causative gene for a familial form of Parkinson's disease. DJ-1 has multiple functions, including anti-oxidative stress reaction and cysteine 106 (C106) of DJ-1 is an essential amino acid for DJ-1 to exert its function. While increased expression and secretion of DJ-1 into serum in patients with various cancers and regulation of p53 and ... [more]

Int. J. Oncol. Dec. 01, 2009; 35(6);1331-41 [Pubmed: 19885556]

Throughput

  • Low Throughput

Curated By

  • BioGRID