BAIT
SAM50
OMP85, TOB55, SAM complex subunit SAM50, YNL026W
Component of the Sorting and Assembly Machinery (SAM) complex; the SAM (or TOB) complex is located in the mitochondrial outer membrane; the complex binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85
GO Process (2)
GO Function (1)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
SPG4
YMR107W
Protein required for high temperature survival during stationary phase; not required for growth on nonfermentable carbon sources
GO Process (0)
GO Function (0)
GO Component (0)
Saccharomyces cerevisiae (S288c)
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Coupling of mitochondrial import and export translocases by receptor-mediated supercomplex formation.
The mitochondrial outer membrane harbors two protein translocases that are essential for cell viability: the translocase of the outer mitochondrial membrane (TOM) and the sorting and assembly machinery (SAM). The precursors of β-barrel proteins use both translocases-TOM for import to the intermembrane space and SAM for export into the outer membrane. It is unknown if the translocases cooperate and where ... [more]
Cell Aug. 01, 2013; 154(3);596-608 [Pubmed: 23911324]
Throughput
- High Throughput
Curated By
- BioGRID