Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

FBXL13 directs the proteolysis of CEP192 to regulate centrosome homeostasis and cell migration.

Fung E, Richter C, Yang HB, Schaeffer I, Fischer R, Kessler BM, Bassermann F, D'Angiolella V

Aberrant centrosome organisation with ensuing alterations of microtubule nucleation capacity enables tumour cells to proliferate and invade despite increased genomic instability. CEP192 is a key factor in the initiation process of centrosome duplication and in the control of centrosome microtubule nucleation. However, regulatory means of CEP192 have remained unknown. Here, we report that FBXL13, a binding determinant of SCF (SKP1-CUL1-F-box)-family ... [more]

EMBO Rep. Mar. 01, 2018; 19(3); [Pubmed: 29348145]

Throughput

  • High Throughput

Additional Notes

  • Fbxl13 isoform 1
  • Purification from HEK293T using Flag tagged Fbxl13.

Curated By

  • BioGRID