Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Endocytosis of the glucose transporter GLUT8 is mediated by interaction of a dileucine motif with the beta2-adaptin subunit of the AP-2 adaptor complex.

Schmidt U, Briese S, Leicht K, Schuermann A, Joost HG, Al-Hasani H

The glucose transporter GLUT8 cycles between intracellular vesicles and the plasma membrane. Like the insulin-responsive glucose transporter GLUT4, GLUT8 is primarily located in intracellular compartments under basal conditions. Whereas translocation of GLUT4 to the plasma membrane is stimulated by insulin, the distribution of GLUT8 is not affected by insulin treatment in adipose cells. However, blocking endocytosis by co-expression of a ... [more]

J. Cell. Sci. Jun. 01, 2006; 119(Pt 11);2321-31 [Pubmed: 16723738]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SLC2A8 AP1B1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Schmidt U (2006)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

SLC2A8

Gene Ontology Biological Process

Gene Ontology Cellular Component

AP1B1

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]protein kinase binding [ISS]transporter activity [TAS]