An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

SUMO1 modification of KHSRP regulates tumorigenesis by preventing the TL-G-Rich miRNA biogenesis.

Yuan H, Deng R, Zhao X, Chen R, Hou G, Zhang H, Wang Y, Xu M, Jiang B, Yu J

MicroRNAs (miRNAs) are important regulators involved in diverse physiological and pathological processes including cancer. SUMO (small ubiquitin-like modifier) is a reversible protein modifier. We recently found that SUMOylation of TARBP2 and DGCR8 is involved in the regulation of the miRNA pathway. KHSRP is a single stranded nucleic acid binding protein with roles in transcription and mRNA decay, and it is ... [more]

Mol. Cancer Oct. 11, 2017; 16(1);157 [Pubmed: 29020972]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

KHSRP

Gene Ontology Biological Process

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]

Gene Ontology Cellular Component

DGCR8