BAIT
FAM92A
6720467C03Rik, Fam92a1, RP24-215D6.5
family with sequence similarity 92, member A
GO Process (0)
GO Function (1)
GO Component (0)
Gene Ontology Molecular Function
Mus musculus
PREY
STK36
1700112N14Rik, B930045J24, FU, Fused, mKIAA1278
serine/threonine kinase 36
GO Process (7)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
- brain development [IMP]
- cilium assembly [IDA]
- epithelial cilium movement [IMP]
- positive regulation of hh target transcription factor activity [IDA]
- positive regulation of smoothened signaling pathway [IDA]
- post-embryonic development [IMP]
- regulation of sequence-specific DNA binding transcription factor activity [ISO]
Gene Ontology Molecular Function
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
A CRISPR-based screen for Hedgehog signaling provides insights into ciliary function and ciliopathies.
Primary cilia organize Hedgehog signaling and shape embryonic development, and their dysregulation is the unifying cause of ciliopathies. We conducted a functional genomic screen for Hedgehog signaling by engineering antibiotic-based selection of Hedgehog-responsive cells and applying genome-wide CRISPR-mediated gene disruption. The screen can robustly identify factors required for ciliary signaling with few false positives or false negatives. Characterization of hit ... [more]
Nat. Genet. Mar. 01, 2018; 50(3);460-471 [Pubmed: 29459677]
Throughput
- High Throughput
Curated By
- BioGRID