BAIT
FAM92A
6720467C03Rik, Fam92a1, RP24-215D6.5
family with sequence similarity 92, member A
GO Process (0)
GO Function (1)
GO Component (0)
Gene Ontology Molecular Function
Mus musculus
PREY
ACTN4
C77391
actinin alpha 4
GO Process (7)
GO Function (7)
GO Component (14)
Gene Ontology Biological Process
- actin filament bundle assembly [IDA]
- negative regulation of cellular component movement [IDA]
- positive regulation of cellular component movement [IDA, IMP, ISO]
- positive regulation of pinocytosis [IMP]
- protein localization to tight junction [IMP]
- regulation of apoptotic process [ISO]
- tight junction assembly [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Z disc [IDA]
- cell-cell junction [IDA]
- cortical cytoskeleton [IDA]
- cytoplasm [IDA, ISO]
- extracellular space [ISO]
- extracellular vesicular exosome [ISO]
- focal adhesion [ISO]
- neuron projection [ISO]
- nucleus [ISO]
- perinuclear region of cytoplasm [ISO]
- protein complex [ISO]
- pseudopodium [IDA]
- ribonucleoprotein complex [ISO]
- stress fiber [IDA, ISO]
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
A CRISPR-based screen for Hedgehog signaling provides insights into ciliary function and ciliopathies.
Primary cilia organize Hedgehog signaling and shape embryonic development, and their dysregulation is the unifying cause of ciliopathies. We conducted a functional genomic screen for Hedgehog signaling by engineering antibiotic-based selection of Hedgehog-responsive cells and applying genome-wide CRISPR-mediated gene disruption. The screen can robustly identify factors required for ciliary signaling with few false positives or false negatives. Characterization of hit ... [more]
Nat. Genet. Mar. 01, 2018; 50(3);460-471 [Pubmed: 29459677]
Throughput
- High Throughput
Curated By
- BioGRID