BAIT
TTC23
1600012K10Rik
tetratricopeptide repeat domain 23
GO Process (0)
GO Function (0)
GO Component (0)
Mus musculus
PREY
ANXA2
AW215814, Cal1h, CT009708.1
annexin A2
GO Process (15)
GO Function (8)
GO Component (26)
Gene Ontology Biological Process
- angiogenesis [IMP]
- body fluid secretion [ISO]
- cellular response to acid chemical [IDA]
- collagen fibril organization [IMP]
- fibrinolysis [IMP]
- membrane budding [ISO]
- membrane raft assembly [ISO]
- negative regulation of catalytic activity [ISO]
- osteoclast development [ISO]
- positive regulation of binding [IMP]
- positive regulation of fibroblast proliferation [ISO]
- positive regulation of protein phosphorylation [ISO]
- positive regulation of vesicle fusion [ISO]
- protein heterotetramerization [ISO]
- protein targeting to plasma membrane [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Schmidt-Lanterman incisure [IDA]
- cell cortex [ISO]
- cell junction [ISS]
- cell surface [ISO]
- cytoplasm [IDA, ISO]
- early endosome [IDA]
- endosome [ISO]
- extracellular space [ISO]
- extracellular vesicular exosome [ISO]
- extrinsic component of plasma membrane [IDA]
- late endosome membrane [ISO]
- lipid particle [ISO]
- lysosomal membrane [ISO]
- macropinosome [ISO]
- membrane [ISO, ISS]
- membrane raft [ISO]
- midbody [ISO]
- myelin sheath adaxonal region [IDA]
- nucleus [ISO]
- perinuclear region of cytoplasm [ISO]
- plasma membrane [ISO]
- protein complex [ISO]
- ruffle [ISO]
- sarcolemma [IDA]
- stress fiber [ISS]
- vesicle [ISO]
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
A CRISPR-based screen for Hedgehog signaling provides insights into ciliary function and ciliopathies.
Primary cilia organize Hedgehog signaling and shape embryonic development, and their dysregulation is the unifying cause of ciliopathies. We conducted a functional genomic screen for Hedgehog signaling by engineering antibiotic-based selection of Hedgehog-responsive cells and applying genome-wide CRISPR-mediated gene disruption. The screen can robustly identify factors required for ciliary signaling with few false positives or false negatives. Characterization of hit ... [more]
Nat. Genet. Mar. 01, 2018; 50(3);460-471 [Pubmed: 29459677]
Throughput
- High Throughput
Curated By
- BioGRID