BAIT

TTC23

1600012K10Rik
tetratricopeptide repeat domain 23
GO Process (0)
GO Function (0)
GO Component (0)
Mus musculus
PREY

EEF1A1

eukaryotic translation elongation factor 1 alpha 1
Mus musculus

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A CRISPR-based screen for Hedgehog signaling provides insights into ciliary function and ciliopathies.

Breslow DK, Hoogendoorn S, Kopp AR, Morgens DW, Vu BK, Kennedy MC, Han K, Li A, Hess GT, Bassik MC, Chen JK, Nachury MV

Primary cilia organize Hedgehog signaling and shape embryonic development, and their dysregulation is the unifying cause of ciliopathies. We conducted a functional genomic screen for Hedgehog signaling by engineering antibiotic-based selection of Hedgehog-responsive cells and applying genome-wide CRISPR-mediated gene disruption. The screen can robustly identify factors required for ciliary signaling with few false positives or false negatives. Characterization of hit ... [more]

Nat. Genet. Mar. 01, 2018; 50(3);460-471 [Pubmed: 29459677]

Throughput

  • High Throughput

Curated By

  • BioGRID