BAIT
TTC23
1600012K10Rik
tetratricopeptide repeat domain 23
GO Process (0)
GO Function (0)
GO Component (0)
Mus musculus
PREY
HSP90AA1
86kDa, 89kDa, AL024080, AL024147, Hsp86-1, Hsp89, Hsp90, Hspca, hsp4, RP23-60K23.1
heat shock protein 90, alpha (cytosolic), class A member 1
GO Process (13)
GO Function (20)
GO Component (18)
Gene Ontology Biological Process
- ATP catabolic process [ISO]
- chaperone-mediated protein complex assembly [ISO]
- neuron migration [ISO]
- nitric oxide biosynthetic process [TAS]
- positive regulation of cell size [ISO]
- positive regulation of cytotoxic T cell differentiation [TAS]
- positive regulation of lamellipodium assembly [ISO]
- positive regulation of nitric oxide biosynthetic process [IDA]
- positive regulation of protein import into nucleus, translocation [ISO]
- protein import into mitochondrial outer membrane [ISO]
- protein refolding [TAS]
- regulation of catalytic activity [ISO]
- response to unfolded protein [TAS]
Gene Ontology Molecular Function- ATP binding [ISO]
- ATPase activity [ISO]
- CTP binding [ISO]
- GTP binding [ISO]
- MHC class II protein complex binding [ISO]
- Rho GDP-dissociation inhibitor binding [ISO]
- TPR domain binding [ISO]
- UTP binding [ISO]
- dATP binding [ISO]
- identical protein binding [ISO]
- ion channel binding [ISO]
- mRNA binding [ISO]
- nitric-oxide synthase regulator activity [IDA, ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- protein kinase binding [ISO]
- protein phosphatase binding [ISO]
- sulfonylurea receptor binding [ISO]
- unfolded protein binding [TAS]
- ATP binding [ISO]
- ATPase activity [ISO]
- CTP binding [ISO]
- GTP binding [ISO]
- MHC class II protein complex binding [ISO]
- Rho GDP-dissociation inhibitor binding [ISO]
- TPR domain binding [ISO]
- UTP binding [ISO]
- dATP binding [ISO]
- identical protein binding [ISO]
- ion channel binding [ISO]
- mRNA binding [ISO]
- nitric-oxide synthase regulator activity [IDA, ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- protein kinase binding [ISO]
- protein phosphatase binding [ISO]
- sulfonylurea receptor binding [ISO]
- unfolded protein binding [TAS]
Gene Ontology Cellular Component
- apical plasma membrane [ISO]
- basolateral plasma membrane [ISO]
- brush border membrane [ISO]
- cell surface [ISO]
- cytoplasm [IDA, ISO]
- cytosol [IDA, ISO, TAS]
- extracellular matrix [ISO]
- extracellular vesicular exosome [ISO]
- intracellular [IDA]
- membrane [ISO]
- mitochondrion [ISO]
- myelin sheath [IDA]
- neuron projection [ISO]
- neuronal cell body [ISO]
- nucleoplasm [ISO]
- nucleus [ISO]
- perinuclear region of cytoplasm [ISO]
- protein complex [ISO]
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
A CRISPR-based screen for Hedgehog signaling provides insights into ciliary function and ciliopathies.
Primary cilia organize Hedgehog signaling and shape embryonic development, and their dysregulation is the unifying cause of ciliopathies. We conducted a functional genomic screen for Hedgehog signaling by engineering antibiotic-based selection of Hedgehog-responsive cells and applying genome-wide CRISPR-mediated gene disruption. The screen can robustly identify factors required for ciliary signaling with few false positives or false negatives. Characterization of hit ... [more]
Nat. Genet. Mar. 01, 2018; 50(3);460-471 [Pubmed: 29459677]
Throughput
- High Throughput
Curated By
- BioGRID