BAIT
TTC23
1600012K10Rik
tetratricopeptide repeat domain 23
GO Process (0)
GO Function (0)
GO Component (0)
Mus musculus
PREY
UBB
AL033289, Rps27a, Uba52, Ubb2, Ubc, RP23-234K24.1
ubiquitin B
GO Process (16)
GO Function (0)
GO Component (4)
Gene Ontology Biological Process
- adipose tissue development [IMP]
- energy homeostasis [IMP]
- fat pad development [IMP]
- female gonad development [IMP]
- female meiosis I [IMP]
- hypothalamus gonadotrophin-releasing hormone neuron development [IMP]
- male gonad development [IMP]
- male meiosis I [IMP]
- mitochondrion transport along microtubule [ISO]
- neuron projection morphogenesis [ISO]
- positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator [ISO]
- regulation of mitochondrial membrane potential [ISO]
- regulation of neuron death [ISO]
- regulation of proteasomal protein catabolic process [ISO]
- seminiferous tubule development [IMP]
- ubiquitin homeostasis [IGI, IMP]
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
A CRISPR-based screen for Hedgehog signaling provides insights into ciliary function and ciliopathies.
Primary cilia organize Hedgehog signaling and shape embryonic development, and their dysregulation is the unifying cause of ciliopathies. We conducted a functional genomic screen for Hedgehog signaling by engineering antibiotic-based selection of Hedgehog-responsive cells and applying genome-wide CRISPR-mediated gene disruption. The screen can robustly identify factors required for ciliary signaling with few false positives or false negatives. Characterization of hit ... [more]
Nat. Genet. Mar. 01, 2018; 50(3);460-471 [Pubmed: 29459677]
Throughput
- High Throughput
Curated By
- BioGRID