BAIT
TTC23
1600012K10Rik
tetratricopeptide repeat domain 23
GO Process (0)
GO Function (0)
GO Component (0)
Mus musculus
PREY
GAPDH
Gapd
glyceraldehyde-3-phosphate dehydrogenase
GO Process (13)
GO Function (4)
GO Component (14)
Gene Ontology Biological Process
- apoptotic process [ISO]
- carbohydrate metabolic process [ISO]
- cellular response to interferon-gamma [ISO]
- gluconeogenesis [ISO]
- glycolytic process [ISO]
- microtubule cytoskeleton organization [ISO]
- multicellular organismal development [IMP]
- negative regulation of translation [ISO]
- neuron apoptotic process [ISO]
- oxidation-reduction process [IMP]
- peptidyl-cysteine S-trans-nitrosylation [ISO]
- protein stabilization [ISO]
- response to ammonium ion [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- GAIT complex [IDA, ISO]
- cytoplasm [IDA, ISO]
- cytosol [IDA, ISO]
- extracellular vesicular exosome [ISO]
- intracellular membrane-bounded organelle [ISO]
- lipid particle [ISO]
- membrane [ISO]
- microtubule cytoskeleton [ISO]
- mitochondrion [IDA]
- nuclear membrane [ISO]
- nucleus [ISO]
- plasma membrane [ISO]
- ribonucleoprotein complex [ISO]
- vesicle [ISO]
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
A CRISPR-based screen for Hedgehog signaling provides insights into ciliary function and ciliopathies.
Primary cilia organize Hedgehog signaling and shape embryonic development, and their dysregulation is the unifying cause of ciliopathies. We conducted a functional genomic screen for Hedgehog signaling by engineering antibiotic-based selection of Hedgehog-responsive cells and applying genome-wide CRISPR-mediated gene disruption. The screen can robustly identify factors required for ciliary signaling with few false positives or false negatives. Characterization of hit ... [more]
Nat. Genet. Mar. 01, 2018; 50(3);460-471 [Pubmed: 29459677]
Throughput
- High Throughput
Curated By
- BioGRID