BAIT
1600002H07RIK
RIKEN cDNA 1600002H07 gene
GO Process (0)
GO Function (0)
GO Component (0)
Mus musculus
PREY
CRYAB
Crya-2, Crya2, HspB5, P23
crystallin, alpha B
GO Process (19)
GO Function (7)
GO Component (14)
Gene Ontology Biological Process
- apoptotic process involved in morphogenesis [IGI]
- camera-type eye development [IGI]
- cellular response to gamma radiation [ISO]
- lens development in camera-type eye [IGI]
- microtubule polymerization or depolymerization [ISO]
- muscle organ development [IGI]
- negative regulation of apoptotic process [IMP, ISO]
- negative regulation of cell growth [ISO]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [IGI]
- negative regulation of gene expression [IMP]
- negative regulation of intracellular transport [ISO]
- negative regulation of reactive oxygen species metabolic process [ISO]
- protein folding [ISO]
- protein homooligomerization [ISO]
- regulation of cell death [ISO]
- response to hydrogen peroxide [IMP]
- response to hypoxia [IMP]
- stress-activated MAPK cascade [ISO]
- tubulin complex assembly [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
A CRISPR-based screen for Hedgehog signaling provides insights into ciliary function and ciliopathies.
Primary cilia organize Hedgehog signaling and shape embryonic development, and their dysregulation is the unifying cause of ciliopathies. We conducted a functional genomic screen for Hedgehog signaling by engineering antibiotic-based selection of Hedgehog-responsive cells and applying genome-wide CRISPR-mediated gene disruption. The screen can robustly identify factors required for ciliary signaling with few false positives or false negatives. Characterization of hit ... [more]
Nat. Genet. Mar. 01, 2018; 50(3);460-471 [Pubmed: 29459677]
Throughput
- High Throughput
Curated By
- BioGRID