BAIT
1600002H07RIK
RIKEN cDNA 1600002H07 gene
GO Process (0)
GO Function (0)
GO Component (0)
Mus musculus
PREY
TFRC
2610028K12Rik, AI195355, AI426448, AU015758, CD71, E430033M20Rik, Mtvr-1, Mtvr1, TFR, TFR1, TR, Trfr, p90
transferrin receptor
GO Process (7)
GO Function (8)
GO Component (17)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- blood microparticle [ISO]
- cell [IMP]
- cell surface [IDA, ISO]
- coated pit [IDA, ISO]
- cytoplasmic membrane-bounded vesicle [ISO]
- endosome [IDA, ISO]
- external side of plasma membrane [IDA]
- extracellular region [ISO]
- extracellular space [ISO]
- extracellular vesicular exosome [IDA, ISO]
- intracellular membrane-bounded organelle [ISO]
- mitochondrion [ISO]
- perinuclear region of cytoplasm [IDA]
- plasma membrane [IDA, ISO]
- recycling endosome [IDA, ISO]
- recycling endosome membrane [ISO]
- vesicle [ISO]
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
A CRISPR-based screen for Hedgehog signaling provides insights into ciliary function and ciliopathies.
Primary cilia organize Hedgehog signaling and shape embryonic development, and their dysregulation is the unifying cause of ciliopathies. We conducted a functional genomic screen for Hedgehog signaling by engineering antibiotic-based selection of Hedgehog-responsive cells and applying genome-wide CRISPR-mediated gene disruption. The screen can robustly identify factors required for ciliary signaling with few false positives or false negatives. Characterization of hit ... [more]
Nat. Genet. Mar. 01, 2018; 50(3);460-471 [Pubmed: 29459677]
Throughput
- High Throughput
Curated By
- BioGRID