MAP3K5
Gene Ontology Biological Process
- JNK cascade [IDA]
- MAPK cascade [IDA]
- activation of JUN kinase activity [TAS]
- activation of MAPKK activity [IDA]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- apoptotic signaling pathway [TAS]
- cellular response to hydrogen peroxide [IDA]
- intrinsic apoptotic signaling pathway in response to oxidative stress [IDA]
- positive regulation of apoptotic process [IDA]
- positive regulation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- positive regulation of neuron death [IGI]
- protein phosphorylation [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CIB1
Gene Ontology Biological Process
- apoptotic process [IMP]
- cellular response to DNA damage stimulus [IDA]
- cellular response to growth factor stimulus [ISS]
- cellular response to nerve growth factor stimulus [IDA]
- cellular response to tumor necrosis factor [IMP]
- cytoplasmic microtubule organization [IMP]
- double-strand break repair [TAS]
- endomitotic cell cycle [IDA]
- extrinsic apoptotic signaling pathway [TAS]
- negative regulation of apoptotic process [IMP]
- negative regulation of cell proliferation [IMP]
- negative regulation of megakaryocyte differentiation [ISS]
- negative regulation of microtubule depolymerization [IDA]
- negative regulation of neuron projection development [IDA]
- negative regulation of protein kinase B signaling [ISS]
- negative regulation of protein phosphorylation [ISS]
- platelet formation [ISS]
- positive regulation of ERK1 and ERK2 cascade [ISS]
- positive regulation of NF-kappaB transcription factor activity [IMP]
- positive regulation of calcineurin-NFAT signaling cascade [IDA]
- positive regulation of cell adhesion mediated by integrin [IDA]
- positive regulation of cell growth [ISS]
- positive regulation of cell migration [ISS]
- positive regulation of cell migration involved in sprouting angiogenesis [ISS]
- positive regulation of cell proliferation [ISS]
- positive regulation of cell-matrix adhesion [ISS]
- positive regulation of establishment of protein localization to plasma membrane [IGI]
- positive regulation of male germ cell proliferation [ISS]
- positive regulation of metalloenzyme activity [ISS]
- positive regulation of protein phosphorylation [ISS]
- positive regulation of protein serine/threonine kinase activity [ISS]
- positive regulation of protein targeting to membrane [IMP]
- positive regulation of substrate adhesion-dependent cell spreading [IDA]
- regulation of cell division [IMP]
- regulation of cell proliferation [IMP]
- response to ischemia [ISS]
- spermatid development [ISS]
- thrombopoietin-mediated signaling pathway [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cell periphery [IDA]
- centrosome [IDA]
- cytoplasm [IDA, IMP]
- endoplasmic reticulum [IMP]
- extracellular vesicular exosome [IDA]
- filopodium tip [IDA]
- growth cone [IDA]
- lamellipodium [IDA]
- membrane [IDA]
- neuron projection [IDA]
- neuronal cell body [IDA]
- nucleoplasm [IDA, IMP]
- nucleus [IDA]
- perinuclear region of cytoplasm [IDA]
- plasma membrane [IDA]
- sarcolemma [IDA]
- vesicle [IDA]
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
CIB1 functions as a Ca(2+)-sensitive modulator of stress-induced signaling by targeting ASK1.
Calcium and integrin binding protein 1 (CIB1) is a Ca(2+)-binding protein of 22 kDa that was initially identified as a protein that interacts with integrin alpha(IIb). Although it interacts with various proteins and has been implicated in diverse cellular functions, the molecular mechanism by which CIB1 regulates intracellular signaling networks has remained unclear. We now show that, by targeting apoptosis ... [more]
Throughput
- Low Throughput
Additional Notes
- Source of CIB1 not clear
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| MAP3K5 CIB1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| CIB1 MAP3K5 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| MAP3K5 CIB1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| CIB1 MAP3K5 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - | |
| CIB1 MAP3K5 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | 2392124 |
Curated By
- BioGRID