An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Homer protein increases activation of Ca2+ sparks in permeabilized skeletal muscle.

Ward CW, Feng W, Tu J, Pessah IN, Worley PK, Schneider MF

Members of the Homer family of proteins are known to form multimeric complexes capable of cross-linking plasma membrane channels (e.g. metabotropic glutamate receptor) and intracellular Ca2+ release channels (e.g. inositol trisphosphate receptor) in neurons, which potentiates Ca2+ release. Recent work has demonstrated direct interaction of Homer proteins with type 1 and type 2 ryanodine receptor (RyR) isoforms. Moreover, Homer proteins ... [more]

J. Biol. Chem. Feb. 13, 2004; 279(7);5781-7 [Pubmed: 14660561]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

HOMER1

Gene Ontology Biological Process

Gene Ontology Molecular Function

G-protein coupled glutamate receptor binding [IBA]ion channel binding [IPI]signaling adaptor activity [IC]

Gene Ontology Cellular Component