BAIT

BFR1

L000000174, YOR198C
Component of mRNP complexes associated with polyribosomes; involved in localization of mRNAs to P bodies; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity
Saccharomyces cerevisiae (S288c)
PREY

AGP1

YCC5, amino acid transporter AGP1, L000003271, YCL025C
Low-affinity amino acid permease with broad substrate range; involved in uptake of asparagine, glutamine, and other amino acids; expression regulated by SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p); AGP1 has a paralog, GNP1, that arose from the whole genome duplication
GO Process (2)
GO Function (2)
GO Component (2)
Saccharomyces cerevisiae (S288c)

Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

Protein-RNA networks revealed through covalent RNA marks.

Lapointe CP, Wilinski D, Saunders HA, Wickens M

Protein-RNA networks are ubiquitous and central in biological control. We present an approach termed RNA Tagging that enables the user to identify protein-RNA interactions in vivo by analyzing purified cellular RNA, without protein purification or cross-linking. An RNA-binding protein of interest is fused to an enzyme that adds uridines to the end of RNA. RNA targets bound by the chimeric ... [more]

Nat. Methods Dec. 01, 2015; 12(12);1163-70 [Pubmed: 26524240]

Throughput

  • High Throughput

Additional Notes

  • RNA Tagging

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
AGP1 BFR1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-6.2615BioGRID
206389
BFR1 AGP1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-6.2615BioGRID
206941

Curated By

  • BioGRID