BAIT

BFR1

L000000174, YOR198C
Component of mRNP complexes associated with polyribosomes; involved in localization of mRNAs to P bodies; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity
Saccharomyces cerevisiae (S288c)
PREY

CPR2

CYP2, peptidylprolyl isomerase CPR2, L000000463, YHR057C
Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway; CPR2 has a paralog, CPR5, that arose from the whole genome duplication; suppresses toxicity of slow-folding human Z-type alpha1-antitrypsin variant associated with liver cirrhosis and emphysema
GO Process (0)
GO Function (1)
GO Component (0)

Gene Ontology Molecular Function

Saccharomyces cerevisiae (S288c)

Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

Protein-RNA networks revealed through covalent RNA marks.

Lapointe CP, Wilinski D, Saunders HA, Wickens M

Protein-RNA networks are ubiquitous and central in biological control. We present an approach termed RNA Tagging that enables the user to identify protein-RNA interactions in vivo by analyzing purified cellular RNA, without protein purification or cross-linking. An RNA-binding protein of interest is fused to an enzyme that adds uridines to the end of RNA. RNA targets bound by the chimeric ... [more]

Nat. Methods Dec. 01, 2015; 12(12);1163-70 [Pubmed: 26524240]

Throughput

  • High Throughput

Additional Notes

  • RNA Tagging

Curated By

  • BioGRID