BAIT
BFR1
L000000174, YOR198C
Component of mRNP complexes associated with polyribosomes; involved in localization of mRNAs to P bodies; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity
GO Process (4)
GO Function (2)
GO Component (5)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
GPR1
L000004298, YDL035C
Plasma membrane G protein coupled receptor (GPCR); interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis
GO Process (9)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [IMP, IPI]
- detection of glucose [IMP]
- detection of sucrose stimulus [IMP]
- glucose mediated signaling pathway [IGI, IMP]
- hexose mediated signaling [IGI, IMP]
- invasive growth in response to glucose limitation [IMP]
- pseudohyphal growth [IMP]
- replicative cell aging [IMP]
- sucrose mediated signaling [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Affinity Capture-RNA
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.
Publication
Protein-RNA networks revealed through covalent RNA marks.
Protein-RNA networks are ubiquitous and central in biological control. We present an approach termed RNA Tagging that enables the user to identify protein-RNA interactions in vivo by analyzing purified cellular RNA, without protein purification or cross-linking. An RNA-binding protein of interest is fused to an enzyme that adds uridines to the end of RNA. RNA targets bound by the chimeric ... [more]
Nat. Methods Dec. 01, 2015; 12(12);1163-70 [Pubmed: 26524240]
Throughput
- High Throughput
Additional Notes
- RNA Tagging
Curated By
- BioGRID