BAIT
BRAF
9930012E13Rik, AA120551, AA387315, AA473386, B-raf, Braf-2, Braf2, C230098H17, C87398, D6Ertd631e
Braf transforming gene
GO Process (27)
GO Function (8)
GO Component (7)
Gene Ontology Biological Process
- CD4-positive, alpha-beta T cell differentiation [IMP]
- MAPK cascade [ISO]
- activation of MAPKK activity [ISO]
- alpha-beta T cell differentiation [IMP]
- cell differentiation [IGI]
- cellular response to calcium ion [ISO]
- cellular response to drug [IDA]
- long-term synaptic potentiation [IMP]
- myeloid progenitor cell differentiation [IMP]
- negative regulation of apoptotic process [ISO]
- negative regulation of endothelial cell apoptotic process [IMP]
- negative regulation of fibroblast migration [IMP]
- negative regulation of neuron apoptotic process [IMP, ISO]
- negative regulation of synaptic vesicle exocytosis [IMP]
- positive T cell selection [IMP]
- positive regulation of ERK1 and ERK2 cascade [IDA, IMP, ISO]
- positive regulation of gene expression [ISO]
- positive regulation of peptidyl-serine phosphorylation [ISO]
- positive regulation of stress fiber assembly [IGI, IMP]
- positive regulation of substrate adhesion-dependent cell spreading [IMP]
- protein heterooligomerization [ISO]
- protein phosphorylation [IDA, ISO]
- regulation of cell proliferation [IGI]
- response to cAMP [ISO]
- response to peptide hormone [ISO]
- somatic stem cell maintenance [IGI]
- visual learning [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
IQGAP1
AA682088, D7Ertd237e, D7Ertd257e, mKIAA0051
IQ motif containing GTPase activating protein 1
GO Process (12)
GO Function (9)
GO Component (21)
Gene Ontology Biological Process
- cellular response to calcium ion [ISO]
- cellular response to epidermal growth factor stimulus [IMP, ISO]
- cellular response to fibroblast growth factor stimulus [IMP]
- cellular response to platelet-derived growth factor stimulus [IMP]
- epidermal growth factor receptor signaling pathway [IMP, ISO]
- fibroblast growth factor receptor signaling pathway [IMP]
- negative regulation of dephosphorylation [IGI]
- neuron projection extension [ISO]
- platelet-derived growth factor receptor signaling pathway [IMP]
- positive regulation of protein kinase activity [IMP, ISO]
- positive regulation of protein serine/threonine kinase activity [ISO]
- regulation of cytokine production [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- actin cytoskeleton [ISO]
- axon [ISO]
- cell junction [ISO]
- cell leading edge [IGI]
- cell-cell junction [IDA]
- cytoplasm [IDA, ISO]
- cytoplasmic ribonucleoprotein granule [ISO]
- extracellular vesicular exosome [ISO]
- extrinsic component of cytoplasmic side of plasma membrane [ISO]
- focal adhesion [ISO]
- growth cone [ISO]
- lateral plasma membrane [IDA]
- membrane [IDA]
- microtubule [ISO]
- microtubule cytoskeleton [ISO]
- midbody [ISO]
- neuron projection [IGI, ISO]
- nucleoplasm [ISO]
- plasma membrane [ISO]
- protein complex [ISO]
- ribonucleoprotein complex [IDA, IGI]
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Phospho-proteomic analyses of B-Raf protein complexes reveal new regulatory principles.
B-Raf represents a critical physiological regulator of the Ras/RAF/MEK/ERK-pathway and a pharmacological target of growing clinical relevance, in particular in oncology. To understand how B-Raf itself is regulated, we combined mass spectrometry with genetic approaches to map its interactome in MCF-10A cells as well as in B-Raf deficient murine embryonic fibroblasts (MEFs) and B-Raf/Raf-1 double deficient DT40 lymphoma cells complemented ... [more]
Oncotarget May. 03, 2016; 7(18);26628-52 [Pubmed: 27034005]
Throughput
- High Throughput
Curated By
- BioGRID