BRAF
Gene Ontology Biological Process
- CD4-positive, alpha-beta T cell differentiation [IMP]
- MAPK cascade [ISO]
- activation of MAPKK activity [ISO]
- alpha-beta T cell differentiation [IMP]
- cell differentiation [IGI]
- cellular response to calcium ion [ISO]
- cellular response to drug [IDA]
- long-term synaptic potentiation [IMP]
- myeloid progenitor cell differentiation [IMP]
- negative regulation of apoptotic process [ISO]
- negative regulation of endothelial cell apoptotic process [IMP]
- negative regulation of fibroblast migration [IMP]
- negative regulation of neuron apoptotic process [IMP, ISO]
- negative regulation of synaptic vesicle exocytosis [IMP]
- positive T cell selection [IMP]
- positive regulation of ERK1 and ERK2 cascade [IDA, IMP, ISO]
- positive regulation of gene expression [ISO]
- positive regulation of peptidyl-serine phosphorylation [ISO]
- positive regulation of stress fiber assembly [IGI, IMP]
- positive regulation of substrate adhesion-dependent cell spreading [IMP]
- protein heterooligomerization [ISO]
- protein phosphorylation [IDA, ISO]
- regulation of cell proliferation [IGI]
- response to cAMP [ISO]
- response to peptide hormone [ISO]
- somatic stem cell maintenance [IGI]
- visual learning [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
YWHAQ
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Phospho-proteomic analyses of B-Raf protein complexes reveal new regulatory principles.
B-Raf represents a critical physiological regulator of the Ras/RAF/MEK/ERK-pathway and a pharmacological target of growing clinical relevance, in particular in oncology. To understand how B-Raf itself is regulated, we combined mass spectrometry with genetic approaches to map its interactome in MCF-10A cells as well as in B-Raf deficient murine embryonic fibroblasts (MEFs) and B-Raf/Raf-1 double deficient DT40 lymphoma cells complemented ... [more]
Throughput
- High Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| BRAF YWHAQ | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID