BAIT
BRAF
9930012E13Rik, AA120551, AA387315, AA473386, B-raf, Braf-2, Braf2, C230098H17, C87398, D6Ertd631e
Braf transforming gene
GO Process (27)
GO Function (8)
GO Component (7)
Gene Ontology Biological Process
- CD4-positive, alpha-beta T cell differentiation [IMP]
- MAPK cascade [ISO]
- activation of MAPKK activity [ISO]
- alpha-beta T cell differentiation [IMP]
- cell differentiation [IGI]
- cellular response to calcium ion [ISO]
- cellular response to drug [IDA]
- long-term synaptic potentiation [IMP]
- myeloid progenitor cell differentiation [IMP]
- negative regulation of apoptotic process [ISO]
- negative regulation of endothelial cell apoptotic process [IMP]
- negative regulation of fibroblast migration [IMP]
- negative regulation of neuron apoptotic process [IMP, ISO]
- negative regulation of synaptic vesicle exocytosis [IMP]
- positive T cell selection [IMP]
- positive regulation of ERK1 and ERK2 cascade [IDA, IMP, ISO]
- positive regulation of gene expression [ISO]
- positive regulation of peptidyl-serine phosphorylation [ISO]
- positive regulation of stress fiber assembly [IGI, IMP]
- positive regulation of substrate adhesion-dependent cell spreading [IMP]
- protein heterooligomerization [ISO]
- protein phosphorylation [IDA, ISO]
- regulation of cell proliferation [IGI]
- response to cAMP [ISO]
- response to peptide hormone [ISO]
- somatic stem cell maintenance [IGI]
- visual learning [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
CDK2
A630093N05Rik
cyclin-dependent kinase 2
GO Process (12)
GO Function (7)
GO Component (12)
Gene Ontology Biological Process
- G1/S transition of mitotic cell cycle [IDA]
- cellular response to insulin stimulus [ISO]
- histone phosphorylation [ISO]
- positive regulation of DNA-dependent DNA replication initiation [IGI]
- positive regulation of cell proliferation [ISO]
- positive regulation of transcription, DNA-templated [IGI]
- potassium ion transport [IGI]
- protein phosphorylation [IDA]
- regulation of cell cycle [IDA, ISO]
- regulation of gene silencing [ISO]
- response to cAMP [ISO]
- response to electrical stimulus [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Cajal body [ISO]
- X chromosome [IDA]
- Y chromosome [IDA]
- chromosome, telomeric region [IDA]
- condensed chromosome [IDA]
- cyclin-dependent protein kinase holoenzyme complex [IPI, ISO]
- cytoplasm [ISO]
- cytosol [ISO]
- endosome [ISO]
- intracellular membrane-bounded organelle [ISO]
- nucleus [IDA, ISO]
- transcription factor complex [IDA]
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Phospho-proteomic analyses of B-Raf protein complexes reveal new regulatory principles.
B-Raf represents a critical physiological regulator of the Ras/RAF/MEK/ERK-pathway and a pharmacological target of growing clinical relevance, in particular in oncology. To understand how B-Raf itself is regulated, we combined mass spectrometry with genetic approaches to map its interactome in MCF-10A cells as well as in B-Raf deficient murine embryonic fibroblasts (MEFs) and B-Raf/Raf-1 double deficient DT40 lymphoma cells complemented ... [more]
Oncotarget May. 03, 2016; 7(18);26628-52 [Pubmed: 27034005]
Throughput
- High Throughput
Curated By
- BioGRID