BAIT
BRAF
9930012E13Rik, AA120551, AA387315, AA473386, B-raf, Braf-2, Braf2, C230098H17, C87398, D6Ertd631e
Braf transforming gene
GO Process (27)
GO Function (8)
GO Component (7)
Gene Ontology Biological Process
- CD4-positive, alpha-beta T cell differentiation [IMP]
- MAPK cascade [ISO]
- activation of MAPKK activity [ISO]
- alpha-beta T cell differentiation [IMP]
- cell differentiation [IGI]
- cellular response to calcium ion [ISO]
- cellular response to drug [IDA]
- long-term synaptic potentiation [IMP]
- myeloid progenitor cell differentiation [IMP]
- negative regulation of apoptotic process [ISO]
- negative regulation of endothelial cell apoptotic process [IMP]
- negative regulation of fibroblast migration [IMP]
- negative regulation of neuron apoptotic process [IMP, ISO]
- negative regulation of synaptic vesicle exocytosis [IMP]
- positive T cell selection [IMP]
- positive regulation of ERK1 and ERK2 cascade [IDA, IMP, ISO]
- positive regulation of gene expression [ISO]
- positive regulation of peptidyl-serine phosphorylation [ISO]
- positive regulation of stress fiber assembly [IGI, IMP]
- positive regulation of substrate adhesion-dependent cell spreading [IMP]
- protein heterooligomerization [ISO]
- protein phosphorylation [IDA, ISO]
- regulation of cell proliferation [IGI]
- response to cAMP [ISO]
- response to peptide hormone [ISO]
- somatic stem cell maintenance [IGI]
- visual learning [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
MAP2K1
MAPKK1, MEKK1, Mek1, Prkmk1
mitogen-activated protein kinase kinase 1
GO Process (34)
GO Function (11)
GO Component (18)
Gene Ontology Biological Process
- Golgi inheritance [ISO]
- MAPK cascade [IMP, ISO]
- activation of MAPK activity [IMP, ISO, TAS]
- cell cycle arrest [ISO]
- cell motility [IMP]
- cell proliferation [ISO]
- cellular senescence [ISO]
- keratinocyte differentiation [IMP]
- labyrinthine layer development [IMP]
- melanosome transport [ISO]
- mitotic nuclear division [ISO]
- negative regulation of cell proliferation [ISO]
- negative regulation of homotypic cell-cell adhesion [ISO]
- neuron differentiation [IMP, ISO]
- neuron projection morphogenesis [ISO]
- peptidyl-tyrosine dephosphorylation [IMP]
- placenta blood vessel development [IMP]
- positive regulation of Ras GTPase activity [ISO]
- positive regulation of Ras protein signal transduction [ISO]
- positive regulation of cell differentiation [IDA]
- positive regulation of cell migration [ISO]
- positive regulation of gene expression [ISO]
- positive regulation of protein serine/threonine kinase activity [ISO]
- positive regulation of transcription elongation from RNA polymerase II promoter [ISO]
- protein heterooligomerization [ISO]
- protein phosphorylation [IMP, ISO, TAS]
- regulation of Golgi inheritance [TAS]
- regulation of early endosome to late endosome transport [TAS]
- regulation of stress-activated MAPK cascade [TAS]
- regulation of vascular smooth muscle contraction [ISO]
- response to axon injury [ISO]
- response to glucocorticoid [ISO]
- response to oxidative stress [ISO]
- vesicle transport along microtubule [ISO]
Gene Ontology Molecular Function- ATP binding [ISO]
- MAP kinase kinase activity [IMP, ISO]
- Ras GTPase binding [ISO]
- mitogen-activated protein kinase kinase kinase binding [ISO]
- protein C-terminus binding [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein kinase binding [ISO]
- protein serine/threonine kinase activator activity [ISO]
- protein serine/threonine/tyrosine kinase activity [TAS]
- receptor signaling protein tyrosine phosphatase activity [IMP]
- ATP binding [ISO]
- MAP kinase kinase activity [IMP, ISO]
- Ras GTPase binding [ISO]
- mitogen-activated protein kinase kinase kinase binding [ISO]
- protein C-terminus binding [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein kinase binding [ISO]
- protein serine/threonine kinase activator activity [ISO]
- protein serine/threonine/tyrosine kinase activity [TAS]
- receptor signaling protein tyrosine phosphatase activity [IMP]
Gene Ontology Cellular Component
- Golgi apparatus [ISO, TAS]
- axon [ISO]
- cell cortex [ISO]
- cytoplasm [ISO]
- cytosol [ISO, TAS]
- dendrite [ISO]
- dendrite cytoplasm [ISO]
- early endosome [TAS]
- endoplasmic reticulum [ISO]
- extracellular vesicular exosome [ISO]
- focal adhesion [TAS]
- late endosome [TAS]
- microtubule [ISO]
- mitochondrion [TAS]
- nucleus [TAS]
- perikaryon [ISO]
- perinuclear region of cytoplasm [ISO]
- plasma membrane [ISO]
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Phospho-proteomic analyses of B-Raf protein complexes reveal new regulatory principles.
B-Raf represents a critical physiological regulator of the Ras/RAF/MEK/ERK-pathway and a pharmacological target of growing clinical relevance, in particular in oncology. To understand how B-Raf itself is regulated, we combined mass spectrometry with genetic approaches to map its interactome in MCF-10A cells as well as in B-Raf deficient murine embryonic fibroblasts (MEFs) and B-Raf/Raf-1 double deficient DT40 lymphoma cells complemented ... [more]
Oncotarget May. 03, 2016; 7(18);26628-52 [Pubmed: 27034005]
Throughput
- High Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| BRAF MAP2K1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID