An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Mass spectrometry analyses of normal and polyglutamine expanded ataxin-3 reveal novel interaction partners involved in mitochondrial function.

Kristensen LV, Oppermann FS, Rauen MJ, Fog K, Schmidt T, Schmidt J, Harmuth T, Hartmann-Petersen R, Thirstrup K

Deubiquitinating enzymes (DUBs) play important roles in a variety of cellular processes, including regulation of protein homeostasis. The DUB ataxin-3 is an enzyme implicated in protein quality control mechanisms. In the neurodegenerative disease spinocerebellar ataxia type 3 (SCA3), ataxin-3 contains an expanded polyglutamine (polyQ) stretch that leads to aggregation of the protein and neuronal dysfunction. Increasing the understanding of ataxin-3 ... [more]

Neurochem. Int. Jan. 01, 2018; 112();5-17 [Pubmed: 29111377]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
ATXN3 HSPA4L	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Weishaeupl D (2019)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

ATXN3

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATPase binding [IPI]Lys48-specific deubiquitinase activity [IDA]Lys63-specific deubiquitinase activity [IDA]identical protein binding [IPI]protein binding [IPI]ubiquitin protein ligase binding [IPI, ISS]ubiquitin thiolesterase activity [ISS]ubiquitin-specific protease activity [ISS]

Gene Ontology Cellular Component

HSPA4L