SHANK3
Gene Ontology Biological Process
- MAPK cascade [ISO, ISS]
- N-methyl-D-aspartate receptor clustering [ISO, ISS]
- adult behavior [ISO]
- alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor clustering [ISO, ISS]
- brain morphogenesis [ISO, ISS]
- dendritic spine morphogenesis [ISO, ISS]
- embryonic epithelial tube formation [ISO]
- guanylate kinase-associated protein clustering [ISO, ISS]
- learning [ISO, ISS]
- locomotory exploration behavior [ISO, ISS]
- memory [ISO, ISS]
- negative regulation of actin filament bundle assembly [ISO, ISS]
- negative regulation of cell volume [ISO, ISS]
- neuromuscular process controlling balance [ISO, ISS]
- positive regulation of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity [ISO, ISS]
- positive regulation of dendritic spine development [ISO, ISS]
- positive regulation of excitatory postsynaptic membrane potential [ISO, ISS]
- positive regulation of glutamate receptor signaling pathway [IMP, ISO, ISS]
- positive regulation of long-term neuronal synaptic plasticity [ISO, ISS]
- positive regulation of synapse structural plasticity [ISO, ISS]
- positive regulation of synaptic transmission, glutamatergic [ISO, ISS]
- postsynaptic density assembly [ISO, ISS]
- protein oligomerization [IDA]
- regulation of behavioral fear response [ISO, ISS]
- regulation of dendritic spine morphogenesis [ISO, ISS]
- regulation of grooming behavior [ISO, ISS]
- regulation of long term synaptic depression [ISO, ISS]
- regulation of long-term synaptic potentiation [ISO, ISS]
- social behavior [ISO, ISS]
- striatal medium spiny neuron differentiation [ISO, ISS]
- synapse assembly [ISO, ISS]
- vocal learning [ISO]
- vocalization behavior [ISO, ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
HRAS
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- MAPK cascade [TAS]
- Ras protein signal transduction [IDA, TAS]
- activation of MAPKK activity [TAS]
- axon guidance [TAS]
- blood coagulation [TAS]
- cell cycle arrest [IDA, IMP]
- cell surface receptor signaling pathway [TAS]
- cellular senescence [IDA]
- chemotaxis [TAS]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- innate immune response [TAS]
- insulin receptor signaling pathway [TAS]
- leukocyte migration [TAS]
- mitotic cell cycle checkpoint [IDA]
- negative regulation of Rho GTPase activity [IDA]
- negative regulation of cell proliferation [IDA]
- negative regulation of gene expression [IDA]
- neurotrophin TRK receptor signaling pathway [TAS]
- organ morphogenesis [TAS]
- positive regulation of DNA replication [IDA]
- positive regulation of ERK1 and ERK2 cascade [IDA]
- positive regulation of JNK cascade [IDA]
- positive regulation of MAP kinase activity [IDA]
- positive regulation of MAPK cascade [IDA]
- positive regulation of Rac GTPase activity [IDA]
- positive regulation of actin cytoskeleton reorganization [IDA]
- positive regulation of cell migration [IDA]
- positive regulation of cell proliferation [IDA]
- positive regulation of epithelial cell proliferation [IMP]
- positive regulation of miRNA metabolic process [IDA]
- positive regulation of protein phosphorylation [IDA]
- positive regulation of ruffle assembly [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of wound healing [IDA]
- signal transduction [NAS]
- small GTPase mediated signal transduction [TAS]
- synaptic transmission [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
SHANK proteins limit integrin activation by directly interacting with Rap1 and R-Ras.
SHANK3, a synaptic scaffold protein and actin regulator, is widely expressed outside of the central nervous system with predominantly unknown function. Solving the structure of the SHANK3 N-terminal region revealed that the SPN domain is an unexpected Ras-association domain with high affinity for GTP-bound Ras and Rap G-proteins. The role of Rap1 in integrin activation is well established but the ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SHANK3 HRAS | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID