BAIT

POM34

YLR018C
Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication
Saccharomyces cerevisiae (S288c)
PREY

NUP120

RAT2, L000003138, YKL057C
Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160
Saccharomyces cerevisiae (S288c)

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

The integral membrane protein Pom34p functionally links nucleoporin subcomplexes.

Miao M, Ryan KJ, Wente SR

Here we have examined the function of Pom34p, a novel membrane protein in Saccharomyces cerevisiae, localized to nuclear pore complexes (NPCs). Membrane topology analysis revealed that Pom34p is a double-pass transmembrane protein with both the amino (N) and carboxy (C) termini positioned on the cytosolic/pore face. The network of genetic interactions between POM34 and genes encoding other nucleoporins was established ... [more]

Genetics Mar. 01, 2006; 172(3);1441-57 [Pubmed: 16361228]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: temperature sensitive growth (APO:0000092)
  • phenotype: viability (APO:0000111)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
POM34 NUP120
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High2BioGRID
3619761
NUP120 POM34
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
166856

Curated By

  • BioGRID