BAIT

LET-756

CELE_C05D11.4, C05D11.4
let-756 encodes an fibroblast growth factor (FGF)-like ligand that is required for progression through early larval development; LET-756 is expressed from late embryogenesis to adulthood, with a peak of expression in larvae; with EGL-17, LET-756 is redundantly required to activate EGL-15/FGFR, which in turn activates protein degradation in adult muscle cells; homozygotes for partial loss-of-function alleles are small, clear, and scrawny, but viable, while those for a null allele arrest in early larval development.
Caenorhabditis elegans
PREY

PAR-5

CELE_M117.2
Protein PAR-5
Caenorhabditis elegans

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Direct and heterologous approaches to identify the LET-756/FGF interactome.

Popovici C, Berda Y, Conchonaud F, Harbis A, Birnbaum D, Roubin R

BACKGROUND: Fibroblast growth factors (FGFs) are multifunctional proteins that play important roles in cell communication, proliferation and differentiation. However, many aspects of their activities are not well defined. LET-756, one of the two C. elegans FGFs, is expressed throughout development and is essential for worm development. It is both expressed in the nucleus and secreted. RESULTS: To identify nuclear factors ... [more]

BMC Genomics May. 05, 2006; 7(0);105 [Pubmed: 16672054]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
LET-756 PAR-5
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High3.4444BioGRID
466779

Curated By

  • BioGRID