CNOT8
Gene Ontology Biological Process
- RNA metabolic process [TAS]
- RNA phosphodiester bond hydrolysis, exonucleolytic [IDA]
- exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay [IDA]
- gene expression [TAS]
- gene silencing by miRNA [TAS]
- mRNA metabolic process [TAS]
- negative regulation of cell proliferation [TAS]
- nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [TAS]
- nuclear-transcribed mRNA poly(A) tail shortening [TAS]
- positive regulation of cell proliferation [IMP]
- regulation of transcription, DNA-templated [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
CNOT1
Gene Ontology Biological Process
- RNA metabolic process [TAS]
- RNA phosphodiester bond hydrolysis, exonucleolytic [IDA]
- gene expression [TAS]
- mRNA metabolic process [TAS]
- negative regulation of intracellular estrogen receptor signaling pathway [IDA]
- negative regulation of retinoic acid receptor signaling pathway [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [TAS]
- nuclear-transcribed mRNA poly(A) tail shortening [TAS]
- positive regulation of cytoplasmic mRNA processing body assembly [IDA]
- positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [IMP]
- positive regulation of nuclear-transcribed mRNA poly(A) tail shortening [IMP]
- regulation of stem cell maintenance [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Large-scale mapping of human protein-protein interactions by mass spectrometry.
Mapping protein-protein interactions is an invaluable tool for understanding protein function. Here, we report the first large-scale study of protein-protein interactions in human cells using a mass spectrometry-based approach. The study maps protein interactions for 338 bait proteins that were selected based on known or suspected disease and functional associations. Large-scale immunoprecipitation of Flag-tagged versions of these proteins followed by ... [more]
Quantitative Score
- 0.899 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- cell line: hek-293 cell (BTO:0000007)
Additional Notes
- Exogenous expression of bait
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CNOT1 CNOT8 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 1449315 | |
| CNOT1 CNOT8 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| CNOT1 CNOT8 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| CNOT8 CNOT1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| CNOT1 CNOT8 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID