BAIT
IKBKE
IKK-E, IKK-i, IKKE, IKKI, RP11-534L20.1
inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon
GO Process (12)
GO Function (2)
GO Component (6)
Gene Ontology Biological Process
- MyD88-independent toll-like receptor signaling pathway [TAS]
- NIK/NF-kappaB signaling [IDA]
- TRIF-dependent toll-like receptor signaling pathway [TAS]
- immune response [NAS]
- innate immune response [TAS]
- intrinsic apoptotic signaling pathway in response to DNA damage [IMP]
- negative regulation of type I interferon production [TAS]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IEP]
- protein phosphorylation [IDA]
- toll-like receptor 3 signaling pathway [TAS]
- toll-like receptor 4 signaling pathway [TAS]
- toll-like receptor signaling pathway [TAS]
Gene Ontology Molecular Function
Homo sapiens
PREY
ROCK2
ROCK-II
Rho-associated, coiled-coil containing protein kinase 2
GO Process (14)
GO Function (4)
GO Component (3)
Gene Ontology Biological Process
- axon guidance [TAS]
- centrosome duplication [IMP]
- cytokinesis [NAS]
- negative regulation of angiogenesis [IMP]
- protein phosphorylation [IDA]
- regulation of actin cytoskeleton organization [TAS]
- regulation of cell adhesion [TAS]
- regulation of cell motility [TAS]
- regulation of circadian rhythm [ISS]
- regulation of establishment of cell polarity [TAS]
- regulation of focal adhesion assembly [TAS]
- regulation of keratinocyte differentiation [IMP]
- regulation of stress fiber assembly [TAS]
- smooth muscle contraction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Large-scale mapping of human protein-protein interactions by mass spectrometry.
Mapping protein-protein interactions is an invaluable tool for understanding protein function. Here, we report the first large-scale study of protein-protein interactions in human cells using a mass spectrometry-based approach. The study maps protein interactions for 338 bait proteins that were selected based on known or suspected disease and functional associations. Large-scale immunoprecipitation of Flag-tagged versions of these proteins followed by ... [more]
Mol. Syst. Biol. Mar. 14, 2007; 3(0);89 [Pubmed: 17353931]
Quantitative Score
- 0.716 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- cell line: hek-293 cell (BTO:0000007)
Additional Notes
- Exogenous expression of bait
Curated By
- BioGRID