BAIT
DKK1
DKK-1, SK, UNQ492/PRO1008
dickkopf WNT signaling pathway inhibitor 1
GO Process (13)
GO Function (5)
GO Component (2)
Gene Ontology Biological Process
- extracellular negative regulation of signal transduction [IDA]
- negative regulation of Wnt signaling pathway [IDA]
- negative regulation of canonical Wnt signaling pathway [IDA, IGI]
- negative regulation of canonical Wnt signaling pathway involved in cardiac muscle cell fate commitment [IDA]
- negative regulation of cardiac muscle cell differentiation [IDA]
- negative regulation of mesodermal cell fate specification [IDA]
- negative regulation of pathway-restricted SMAD protein phosphorylation [IDA]
- negative regulation of peptidyl-serine phosphorylation [IDA]
- negative regulation of protein complex assembly [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IDA, ISS]
- positive regulation of heart induction by negative regulation of canonical Wnt signaling pathway [ISS]
- regulation of endodermal cell fate specification [IDA]
- regulation of receptor internalization [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
CORO1A
CLABP, CLIPINA, HCORO1, IMD8, TACO, p57
coronin, actin binding protein, 1A
GO Process (11)
GO Function (7)
GO Component (11)
Gene Ontology Biological Process
- actin cytoskeleton organization [IMP]
- cell-substrate adhesion [IMP]
- cellular component movement [NAS]
- innate immune response [NAS]
- leukocyte chemotaxis [IBA]
- negative regulation of actin nucleation [IDA]
- phagocytosis [IMP]
- phagolysosome assembly [IMP]
- positive chemotaxis [IDA]
- regulation of cell shape [IBA]
- uropod organization [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Large-scale mapping of human protein-protein interactions by mass spectrometry.
Mapping protein-protein interactions is an invaluable tool for understanding protein function. Here, we report the first large-scale study of protein-protein interactions in human cells using a mass spectrometry-based approach. The study maps protein interactions for 338 bait proteins that were selected based on known or suspected disease and functional associations. Large-scale immunoprecipitation of Flag-tagged versions of these proteins followed by ... [more]
Mol. Syst. Biol. Mar. 14, 2007; 3(0);89 [Pubmed: 17353931]
Quantitative Score
- 0.507 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- cell line: hek-293 cell (BTO:0000007)
Additional Notes
- Exogenous expression of bait
Curated By
- BioGRID