YWHAG
Gene Ontology Biological Process
- G2/M transition of mitotic cell cycle [TAS]
- apoptotic process [TAS]
- intrinsic apoptotic signaling pathway [TAS]
- membrane organization [TAS]
- mitotic cell cycle [TAS]
- negative regulation of protein kinase activity [NAS]
- negative regulation of protein serine/threonine kinase activity [NAS]
- positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway [TAS]
- regulation of neuron differentiation [IMP]
- regulation of signal transduction [NAS]
- regulation of synaptic plasticity [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PARD3
Gene Ontology Biological Process
- asymmetric cell division [TAS]
- axonogenesis [TAS]
- cell junction assembly [TAS]
- cell-cell junction organization [TAS]
- establishment of epithelial cell polarity [ISS]
- establishment or maintenance of cell polarity [TAS]
- myelination in peripheral nervous system [ISS]
- negative regulation of peptidyl-threonine phosphorylation [ISS]
- positive regulation of myelination [ISS]
- protein complex assembly [TAS]
- protein kinase C-activating G-protein coupled receptor signaling pathway [TAS]
- protein targeting to membrane [ISS]
- tight junction assembly [ISS, TAS]
- transforming growth factor beta receptor signaling pathway [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Large-scale mapping of human protein-protein interactions by mass spectrometry.
Mapping protein-protein interactions is an invaluable tool for understanding protein function. Here, we report the first large-scale study of protein-protein interactions in human cells using a mass spectrometry-based approach. The study maps protein interactions for 338 bait proteins that were selected based on known or suspected disease and functional associations. Large-scale immunoprecipitation of Flag-tagged versions of these proteins followed by ... [more]
Quantitative Score
- 0.445 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- cell line: hek-293 cell (BTO:0000007)
Additional Notes
- Exogenous expression of bait
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
YWHAG PARD3 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 3376002 | |
YWHAG PARD3 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3236208 | |
YWHAG PARD3 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3111598 | |
YWHAG PARD3 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
YWHAG PARD3 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 3532219 | |
YWHAG PARD3 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | - | BioGRID | 3534987 |
Curated By
- BioGRID