BAIT
HLA-B
AS, HLAB, SPDA1, CDABP0067
major histocompatibility complex, class I, B
GO Process (19)
GO Function (4)
GO Component (13)
Gene Ontology Biological Process
- antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent [IDA]
- antigen processing and presentation of exogenous peptide antigen via MHC class I [TAS]
- antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent [TAS]
- antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent [TAS]
- antigen processing and presentation of peptide antigen via MHC class I [TAS]
- cytokine-mediated signaling pathway [TAS]
- defense response [TAS]
- detection of bacterium [IMP]
- immune response [IMP, NAS]
- innate immune response [TAS]
- interferon-gamma-mediated signaling pathway [TAS]
- positive regulation of T cell mediated cytotoxicity [IDA]
- protection from natural killer cell mediated cytotoxicity [IDA]
- regulation of T cell anergy [IMP]
- regulation of dendritic cell differentiation [IMP]
- regulation of immune response [TAS]
- regulation of interleukin-12 production [IMP]
- regulation of interleukin-6 production [IMP]
- type I interferon signaling pathway [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- ER to Golgi transport vesicle membrane [TAS]
- Golgi apparatus [IDA, ISS]
- Golgi membrane [TAS]
- MHC class I protein complex [IDA, ISS]
- cell surface [IDA, ISS]
- early endosome membrane [TAS]
- endoplasmic reticulum [IDA, ISS]
- extracellular vesicular exosome [IDA]
- integral component of lumenal side of endoplasmic reticulum membrane [TAS]
- integral component of plasma membrane [NAS]
- membrane [IDA, TAS]
- phagocytic vesicle membrane [TAS]
- plasma membrane [TAS]
Homo sapiens
PREY
EZR
CVIL, CVL, HEL-S-105, VIL2
ezrin
GO Process (7)
GO Function (4)
GO Component (18)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- actin cytoskeleton [IDA]
- actin filament [IDA]
- apical part of cell [IDA]
- basolateral plasma membrane [ISS]
- cell periphery [IDA]
- cortical cytoskeleton [TAS]
- cytosol [IDA, TAS]
- extracellular space [IDA]
- extracellular vesicular exosome [IDA]
- extrinsic component of membrane [IDA]
- filopodium [IDA]
- focal adhesion [IDA]
- membrane [IDA]
- microvillus [IDA]
- nucleolus [IDA]
- plasma membrane [IDA]
- ruffle [IDA]
- vesicle [IDA]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Large-scale mapping of human protein-protein interactions by mass spectrometry.
Mapping protein-protein interactions is an invaluable tool for understanding protein function. Here, we report the first large-scale study of protein-protein interactions in human cells using a mass spectrometry-based approach. The study maps protein interactions for 338 bait proteins that were selected based on known or suspected disease and functional associations. Large-scale immunoprecipitation of Flag-tagged versions of these proteins followed by ... [more]
Mol. Syst. Biol. Mar. 14, 2007; 3(0);89 [Pubmed: 17353931]
Quantitative Score
- 0.401 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- hek-293 cell (BTO:0000007)
Additional Notes
- Exogenous expression of bait
Curated By
- BioGRID