BAIT

CHS7

S000007442, YHR142W
Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress
Saccharomyces cerevisiae (S288c)
PREY

CDC1

DSC1, DSR1, ESP2, putative lipid phosphatase CDC1, L000000241, YDR182W
Putative lipid phosphatase of the endoplasmic reticulum; shows Mn2+ dependence and may affect Ca2+ signaling; mutants display actin and general growth defects and pleiotropic defects in cell cycle progression and organelle distribution
GO Process (4)
GO Function (0)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Systematic analysis of complex genetic interactions.

Kuzmin E, VanderSluis B, Wang W, Tan G, Deshpande R, Chen Y, Usaj M, Balint A, Mattiazzi Usaj M, van Leeuwen J, Koch EN, Pons C, Dagilis AJ, Pryszlak M, Wang JZY, Hanchard J, Riggi M, Xu K, Heydari H, San Luis BJ, Shuteriqi E, Zhu H, Van Dyk N, Sharifpoor S, Costanzo M, Loewith R, Caudy A, Bolnick D, Brown GW, Andrews BJ, Boone C, Myers CL

To systematically explore complex genetic interactions, we constructed ~200,000 yeast triple mutants and scored negative trigenic interactions. We selected double-mutant query genes across a broad spectrum of biological processes, spanning a range of quantitative features of the global digenic interaction network and tested for a genetic interaction with a third mutation. Trigenic interactions often occurred among functionally related genes, and ... [more]

Science Apr. 20, 2018; 360(6386); [Pubmed: 29674565]

Quantitative Score

  • -0.174718 [Confidence Score]

Throughput

  • High Throughput

Ontology Terms

  • phenotype: colony size (APO:0000063)

Additional Notes

  • Digenic interaction: Query allele name: chs7-delta+ho-delta; Array allele name: cdc1-1 (GI score = -0.136861, p-value = 0.0476; Digenic)
  • Digenic interactions in this Synthetic genetic array (SGA) analysis were considered to be significant when epsilon > 0.08 and p < 0.05 (positive genetic interaction) and when epsilon < -0.08 and p < 0.05 (negative genetic interaction).
  • Trigenic interaction: Query allele name: chs7-delta+prr1-delta; Array allele name: cdc1-1 (GI score = -0.174718, p-value = 4.20E-8; Modified_A-)
  • Trigenic negative genetic interactions in this triple mutant Synthetic genetic array (SGA) analysis were considered to be significant when tau < -0.08 and p < 0.05.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CHS7 CDC1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1831BioGRID
2048035

Curated By

  • BioGRID