BAIT
EIF4A2
BM-010, DDX2B, EIF4A, EIF4F, eIF-4A-II, eIF4A-II
eukaryotic translation initiation factor 4A2
GO Process (12)
GO Function (4)
GO Component (3)
Gene Ontology Biological Process
- ATP catabolic process [IDA]
- RNA metabolic process [TAS]
- cellular protein metabolic process [TAS]
- cytokine-mediated signaling pathway [TAS]
- gene expression [TAS]
- mRNA metabolic process [TAS]
- negative regulation of RNA-directed RNA polymerase activity [IDA]
- nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay [TAS]
- nuclear-transcribed mRNA poly(A) tail shortening [TAS]
- regulation of translational initiation [TAS]
- translation [TAS]
- translational initiation [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
AKAP17A
721P, AKAP-17A, CCDC133, CXYorf3, DXYS155E, PRKA17A, SFRS17A, XE7, XE7Y, RP13-297E16.1
A kinase (PRKA) anchor protein 17A
GO Process (4)
GO Function (3)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Large-scale mapping of human protein-protein interactions by mass spectrometry.
Mapping protein-protein interactions is an invaluable tool for understanding protein function. Here, we report the first large-scale study of protein-protein interactions in human cells using a mass spectrometry-based approach. The study maps protein interactions for 338 bait proteins that were selected based on known or suspected disease and functional associations. Large-scale immunoprecipitation of Flag-tagged versions of these proteins followed by ... [more]
Mol. Syst. Biol. Mar. 14, 2007; 3(0);89 [Pubmed: 17353931]
Quantitative Score
- 0.344 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- cell line: hek-293 cell (BTO:0000007)
Additional Notes
- Exogenous expression of bait
Curated By
- BioGRID