BAIT
HNF4A
FRTS4, HNF4, HNF4a7, HNF4a8, HNF4a9, HNF4alpha, MODY, MODY1, NR2A1, NR2A21, TCF, TCF14, RP5-1013A22.1
hepatocyte nuclear factor 4, alpha
GO Process (20)
GO Function (9)
GO Component (3)
Gene Ontology Biological Process
- blood coagulation [IDA]
- endocrine pancreas development [TAS]
- gene expression [TAS]
- glucose homeostasis [ISS]
- lipid homeostasis [IMP]
- negative regulation of cell growth [IMP]
- negative regulation of cell proliferation [IMP]
- ornithine metabolic process [IMP]
- phospholipid homeostasis [ISS]
- positive regulation of cholesterol homeostasis [ISS]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- positive regulation of transcription, DNA-templated [IDA]
- regulation of growth hormone receptor signaling pathway [NAS]
- regulation of insulin secretion [ISS]
- regulation of lipid metabolic process [IDA]
- regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- response to glucose [ISS]
- transcription initiation from RNA polymerase II promoter [TAS]
- triglyceride homeostasis [ISS]
- xenobiotic metabolic process [IMP]
Gene Ontology Molecular Function- DNA binding [IDA]
- RNA polymerase II activating transcription factor binding [ISS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISS]
- fatty acid binding [IDA]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- receptor binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription regulatory region DNA binding [IDA]
- DNA binding [IDA]
- RNA polymerase II activating transcription factor binding [ISS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISS]
- fatty acid binding [IDA]
- protein binding [IPI]
- protein homodimerization activity [IDA]
- receptor binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription regulatory region DNA binding [IDA]
Homo sapiens
PREY
PABPC4
APP-1, APP1, PABP4, iPABP
poly(A) binding protein, cytoplasmic 4 (inducible form)
GO Process (4)
GO Function (5)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Large-scale mapping of human protein-protein interactions by mass spectrometry.
Mapping protein-protein interactions is an invaluable tool for understanding protein function. Here, we report the first large-scale study of protein-protein interactions in human cells using a mass spectrometry-based approach. The study maps protein interactions for 338 bait proteins that were selected based on known or suspected disease and functional associations. Large-scale immunoprecipitation of Flag-tagged versions of these proteins followed by ... [more]
Mol. Syst. Biol. Mar. 14, 2007; 3(0);89 [Pubmed: 17353931]
Quantitative Score
- 0.336 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- hek-293 cell (BTO:0000007)
Additional Notes
- Exogenous expression of bait
Curated By
- BioGRID