BAIT
CHL4
CTF17, MCM17, L000000321, YDR254W
Outer kinetochore protein required for chromosome stability; involved in new kinetochore assembly and sister chromatid cohesion; forms a stable complex with Iml3p; peripheral component of the Ctf19 kinetochore complex that interacts with Ctf19p, Ctf3p, and Mif2p; required for the spindle assembly checkpoint; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-N and fission yeast mis15
GO Process (7)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
- chromosome segregation [IMP]
- establishment of meiotic sister chromatid cohesion [IMP]
- establishment of mitotic sister chromatid cohesion [IMP]
- kinetochore assembly [IMP]
- maintenance of meiotic sister chromatid cohesion [IMP]
- mitotic spindle assembly checkpoint [IMP]
- protein localization to chromosome, centromeric region [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
BEM4
ROM7, L000000170, YPL161C
Protein involved in establishment of cell polarity and bud emergence; interacts with the Rho1p small GTP-binding protein and with the Rho-type GTPase Cdc42p; involved in maintenance of proper telomere length
GO Process (3)
GO Function (0)
GO Component (2)
Gene Ontology Biological Process
Saccharomyces cerevisiae (S288c)
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Systematic analysis of complex genetic interactions.
To systematically explore complex genetic interactions, we constructed ~200,000 yeast triple mutants and scored negative trigenic interactions. We selected double-mutant query genes across a broad spectrum of biological processes, spanning a range of quantitative features of the global digenic interaction network and tested for a genetic interaction with a third mutation. Trigenic interactions often occurred among functionally related genes, and ... [more]
Science Apr. 20, 2018; 360(6386); [Pubmed: 29674565]
Quantitative Score
- -0.173903 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
Additional Notes
- Digenic interaction: Query allele name: ho-delta+chl4-delta; Array allele name: bem4-delta (GI score = -0.173903, p-value = 0.0128; Digenic)
- Digenic interactions in this Synthetic genetic array (SGA) analysis were considered to be significant when epsilon > 0.08 and p < 0.05 (positive genetic interaction) and when epsilon < -0.08 and p < 0.05 (negative genetic interaction).
Curated By
- BioGRID