BAIT

MAGED1

DLXIN-1, NRAGE, PRO2292

melanoma antigen family D, 1

GO Process (8)

GO Function (1)

GO Component (3)

Gene Ontology Biological Process

apoptotic process [TAS]

apoptotic signaling pathway [TAS]

circadian regulation of gene expression [ISS]

negative regulation of epithelial cell proliferation [IDA]

neurotrophin TRK receptor signaling pathway [TAS]

positive regulation of apoptotic process [TAS]

regulation of apoptotic process [TAS]

regulation of transcription, DNA-templated [TAS]

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

chromatin [ISS]

protein complex [IDA]

CRISPR Database VEGA HGNC Alliance of Genome Resources OMIM Entrez Gene RefSeq UniprotKB Ensembl HPRD

Homo sapiens

PREY

HSPB1

CMT2F, HEL-S-102, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27

heat shock 27kDa protein 1

Alzheimer's Disease Project

GO Process (21)

GO Function (7)

GO Component (8)

Gene Ontology Biological Process

RNA metabolic process [TAS]

cellular component movement [TAS]

cellular response to vascular endothelial growth factor stimulus [IMP]

gene expression [TAS]

intracellular signal transduction [IMP]

mRNA metabolic process [TAS]

negative regulation of apoptotic process [TAS]

negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway [ISS]

negative regulation of protein kinase activity [ISS]

platelet aggregation [IMP]

positive regulation of angiogenesis [IMP]

positive regulation of blood vessel endothelial cell migration [IMP]

positive regulation of endothelial cell chemotaxis [IMP]

positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway [IMP]

positive regulation of interleukin-1 beta production [ISS]

positive regulation of tumor necrosis factor biosynthetic process [ISS]

regulation of I-kappaB kinase/NF-kappaB signaling [ISS]

regulation of translational initiation [TAS]

response to unfolded protein [NAS]

response to virus [IEP]

retina homeostasis [IEP]

Gene Ontology Molecular Function

identical protein binding [IPI]
poly(A) RNA binding [IDA]
protein binding [IPI]
protein kinase C binding [ISS]
protein kinase C inhibitor activity [ISS]
protein kinase binding [IPI]
ubiquitin binding [ISS]

Gene Ontology Cellular Component

cytoplasm [IDA, TAS]

cytoskeleton [TAS]

extracellular space [IDA]

extracellular vesicular exosome [IDA]

focal adhesion [IDA]

proteasome complex [ISS]

CRISPR Database OMIM HGNC Alliance of Genome Resources VEGA Entrez Gene RefSeq UniprotKB Ensembl HPRD

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Large-scale mapping of human protein-protein interactions by mass spectrometry.

Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D

Mapping protein-protein interactions is an invaluable tool for understanding protein function. Here, we report the first large-scale study of protein-protein interactions in human cells using a mass spectrometry-based approach. The study maps protein interactions for 338 bait proteins that were selected based on known or suspected disease and functional associations. Large-scale immunoprecipitation of Flag-tagged versions of these proteins followed by ... [more]

Mol. Syst. Biol. Mar. 14, 2007; 3(0);89 [Pubmed: 17353931]

Quantitative Score

0.304 [Confidence Score]

Throughput

High Throughput

Ontology Terms

hek-293 cell (BTO:0000007)

Additional Notes

Exogenous expression of bait

Curated By

BioGRID