BAIT

BRF2

BRFU, TFIIIB50, PRO1470
BRF2, RNA polymerase III transcription initiation factor 50 kDa subunit
GO Process (2)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens
PREY

ATAD3A

RP5-832C2.1
ATPase family, AAA domain containing 3A
GO Process (2)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Large-scale mapping of human protein-protein interactions by mass spectrometry.

Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S, McBroom-Cerajewski L, Robinson MD, O'Connor L, Li M, Taylor R, Dharsee M, Ho Y, Heilbut A, Moore L, Zhang S, Ornatsky O, Bukhman YV, Ethier M, Sheng Y, Vasilescu J, Abu-Farha M, Lambert JP, Duewel HS, Stewart II, Kuehl B, Hogue K, Colwill K, Gladwish K, Muskat B, Kinach R, Adams SL, Moran MF, Morin GB, Topaloglou T, Figeys D

Mapping protein-protein interactions is an invaluable tool for understanding protein function. Here, we report the first large-scale study of protein-protein interactions in human cells using a mass spectrometry-based approach. The study maps protein interactions for 338 bait proteins that were selected based on known or suspected disease and functional associations. Large-scale immunoprecipitation of Flag-tagged versions of these proteins followed by ... [more]

Mol. Syst. Biol. Mar. 14, 2007; 3(0);89 [Pubmed: 17353931]

Throughput

  • High Throughput

Ontology Terms

  • cell line: hek-293 cell (BTO:0000007)

Additional Notes

  • Exogenous expression of bait

Curated By

  • BioGRID