BAIT

SRS2

HPR5, DNA helicase SRS2, RADH1, RADH, L000000809, L000001578, YJL092W

DNA helicase and DNA-dependent ATPase; involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability; disassembles Rad51p nucleoprotein filaments during meiotic recombination; functional homolog of human RTEL1

GO Process (6)

GO Function (1)

GO Component (1)

Gene Ontology Biological Process

DNA duplex unwinding [IDA]

DNA repair [IGI]

double-strand break repair via nonhomologous end joining [IDA]

negative regulation of DNA recombination [IMP]

negative regulation of double-strand break repair via homologous recombination [IDA]

protein-DNA complex disassembly [IDA, IMP]

Gene Ontology Molecular Function

DNA helicase activity [IDA, ISS]

Gene Ontology Cellular Component

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

RAD27

ERC11, FEN1, RTH1, multifunctional nuclease RAD27, L000002742, L000000565, YKL113C

5' to 3' exonuclease, 5' flap endonuclease; required for Okazaki fragment processing and maturation, for long-patch base-excision repair and large loop repair (LLR), ribonucleotide excision repair; member of the S. pombe RAD2/FEN1 family; relocalizes to the cytosol in response to hypoxia

GO Process (6)

GO Function (2)

GO Component (3)

Gene Ontology Biological Process

DNA replication, removal of RNA primer [IDA]

base-excision repair, base-free sugar-phosphate removal [IGI, IMP]

double-strand break repair via nonhomologous end joining [IDA]

gene conversion at mating-type locus, DNA repair synthesis [IMP]

maintenance of DNA trinucleotide repeats [IGI, IMP]

replicative cell aging [IMP]

Gene Ontology Molecular Function

5'-3' exonuclease activity [IDA]
5'-flap endonuclease activity [IDA]

Gene Ontology Cellular Component

cytosol [IDA]

mitochondrion [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Systematic analysis of complex genetic interactions.

Kuzmin E, VanderSluis B, Wang W, Tan G, Deshpande R, Chen Y, Usaj M, Balint A, Mattiazzi Usaj M, van Leeuwen J, Koch EN, Pons C, Dagilis AJ, Pryszlak M, Wang JZY, Hanchard J, Riggi M, Xu K, Heydari H, San Luis BJ, Shuteriqi E, Zhu H, Van Dyk N, Sharifpoor S, Costanzo M, Loewith R, Caudy A, Bolnick D, Brown GW, Andrews BJ, Boone C, Myers CL

To systematically explore complex genetic interactions, we constructed ~200,000 yeast triple mutants and scored negative trigenic interactions. We selected double-mutant query genes across a broad spectrum of biological processes, spanning a range of quantitative features of the global digenic interaction network and tested for a genetic interaction with a third mutation. Trigenic interactions often occurred among functionally related genes, and ... [more]

Science Apr. 20, 2018; 360(6386); [Pubmed: 29674565]

Quantitative Score

-0.563837 [Confidence Score]

Throughput

High Throughput

Ontology Terms

phenotype: colony size (APO:0000063)

Additional Notes

Digenic interaction: Query allele name: srs2-delta+ho-delta; Array allele name: rad27-delta (GI score = -0.563837, p-value = 2.45E-37; Digenic)
Digenic interactions in this Synthetic genetic array (SGA) analysis were considered to be significant when epsilon > 0.08 and p < 0.05 (positive genetic interaction) and when epsilon < -0.08 and p < 0.05 (negative genetic interaction).

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RAD27 SRS2	Dosage Lethality Dosage Lethality A genetic interaction is inferred when over expression or increased dosage of one gene causes lethality in a strain that is mutated or deleted for another gene.	Leon Ortiz AM (2011)	Low/High	-	BioGRID	530727
SRS2 RAD27	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2010)	High	-0.3987	BioGRID	390700
RAD27 SRS2	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2010)	High	-0.3987	BioGRID	394108
SRS2 RAD27	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.3486	BioGRID	2135890
RAD27 SRS2	Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.	Dixon SJ (2008)	High	-	BioGRID	341674
SRS2 RAD27	Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.	Pan X (2006)	High	-	BioGRID	455487
SRS2 RAD27	Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.	Tong AH (2004)	High	-	BioGRID	452237
RAD27 SRS2	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Debrauwere H (2001)	Low	-	BioGRID	158936
RAD27 SRS2	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Klein HL (2001)	Low	-	BioGRID	158591
RAD27 SRS2	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Kolesar P (2016)	Low	-	BioGRID	2203529
RAD27 SRS2	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Loeillet S (2005)	Low	-	BioGRID	166753
RAD27 SRS2	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Pan X (2006)	High	-	BioGRID	453378
RAD27 SRS2	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Tong AH (2001)	High	-	BioGRID	109255
SRS2 RAD27	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Tong AH (2001)	High	-	BioGRID	109256
RAD27 SRS2	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Tong AH (2004)	High	-	BioGRID	109257
SRS2 RAD27	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	Xu H (2004)	Low	-	BioGRID	165971
RAD27 SRS2	Synthetic Rescue Synthetic Rescue A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.	Saponaro M (2010)	Low	-	BioGRID	430886

Curated By

BioGRID

Interaction Summary

SRS2

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA helicase activity [IDA, ISS]

Gene Ontology Cellular Component

RAD27

Gene Ontology Biological Process

Gene Ontology Molecular Function

5'-3' exonuclease activity [IDA]5'-flap endonuclease activity [IDA]

Gene Ontology Cellular Component

Negative Genetic

Publication

Systematic analysis of complex genetic interactions.

Quantitative Score

Throughput

Ontology Terms

Additional Notes

Related interactions

Curated By

5'-3' exonuclease activity [IDA]
5'-flap endonuclease activity [IDA]