BAIT

NCOA4

ARA70, ELE1, PTC3, RFG, RP11-481A12.4
nuclear receptor coactivator 4
GO Process (3)
GO Function (2)
GO Component (1)
Homo sapiens
PREY

NR3C1

GR, Gcr, Grl
nuclear receptor subfamily 3, group C, member 1
GO Process (41)
GO Function (25)
GO Component (10)

Gene Ontology Biological Process

Rattus norvegicus

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Interaction of the putative androgen receptor-specific coactivator ARA70/ELE1alpha with multiple steroid receptors and identification of an internally deleted ELE1beta isoform.

Alen P, Claessens F, Schoenmakers E, Swinnen JV, Verhoeven G, Rombauts W, Peeters B

Steroid-regulated gene transcription requires the coordinate physical and functional interaction of hormone receptors, basal transcription factors, and transcriptional coactivators. In this context ARA70, previously called RFG and ELE1, has been described as a putative coactivator that specifically enhances the activity of the androgen receptor (AR) but not that of the glucocorticoid receptor (GR), the progesterone receptor, or the estrogen receptor ... [more]

Mol. Endocrinol. Jan. 01, 1999; 13(1);117-28 [Pubmed: 9892017]

Throughput

  • Low Throughput

Additional Notes

  • ELE1alpha and ELE1beta interact in vitro with the AR, but also with the GR and the ER, in a ligand-independent way

Curated By

  • BioGRID