BAIT
DPH2
L000000522, YKL191W
Protein required for synthesis of diphthamide; required along with Dph1p, Kti11p, Jjj3p, and Dph5p; diphthamide is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph1p and Kti11p
GO Process (1)
GO Function (0)
GO Component (1)
Gene Ontology Biological Process
Saccharomyces cerevisiae (S288c)
PREY
ARP4
ACT3, L000000027, YJL081C
Nuclear actin-related protein involved in chromatin remodeling; component of chromatin-remodeling enzyme complexes
GO Process (7)
GO Function (5)
GO Component (5)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Systematic analysis of complex genetic interactions.
To systematically explore complex genetic interactions, we constructed ~200,000 yeast triple mutants and scored negative trigenic interactions. We selected double-mutant query genes across a broad spectrum of biological processes, spanning a range of quantitative features of the global digenic interaction network and tested for a genetic interaction with a third mutation. Trigenic interactions often occurred among functionally related genes, and ... [more]
Science Apr. 20, 2018; 360(6386); [Pubmed: 29674565]
Quantitative Score
- -0.182722 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
Additional Notes
- Trigenic interaction: Query allele name: dph5-delta+dph2-delta; Array allele name: arp4-G161D (GI score = -0.182722, p-value = 0.0155; Novel)
- Trigenic negative genetic interactions in this triple mutant Synthetic genetic array (SGA) analysis were considered to be significant when tau < -0.08 and p < 0.05.
Curated By
- BioGRID