BAIT
ENT4
YLL038C
Protein of unknown function; contains an N-terminal epsin-like domain; proposed to be involved in the trafficking of Arn1p in the absence of ferrichrome
GO Process (4)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
SRB2
HRS2, MED20, L000002050, L000003586, YHR041C
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance
GO Process (4)
GO Function (5)
GO Component (1)
Gene Ontology Biological Process
- RNA polymerase II transcriptional preinitiation complex assembly [IDA]
- negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to chemical stimulus [IMP]
- negative regulation of ribosomal protein gene transcription from RNA polymerase II promoter in response to nutrient levels [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
Gene Ontology Molecular Function- RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly [IDA]
- RNA polymerase II transcription factor recruiting transcription factor activity [IMP]
- TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA]
- core RNA polymerase II binding transcription factor activity [IDA, IGI]
- protein domain specific binding [IGI]
- RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly [IDA]
- RNA polymerase II transcription factor recruiting transcription factor activity [IMP]
- TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA]
- core RNA polymerase II binding transcription factor activity [IDA, IGI]
- protein domain specific binding [IGI]
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Systematic analysis of complex genetic interactions.
To systematically explore complex genetic interactions, we constructed ~200,000 yeast triple mutants and scored negative trigenic interactions. We selected double-mutant query genes across a broad spectrum of biological processes, spanning a range of quantitative features of the global digenic interaction network and tested for a genetic interaction with a third mutation. Trigenic interactions often occurred among functionally related genes, and ... [more]
Science Apr. 20, 2018; 360(6386); [Pubmed: 29674565]
Quantitative Score
- -0.113498 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
Additional Notes
- Trigenic interaction: Query allele name: ufe1-1+ent4-delta; Array allele name: srb2-delta (GI score = -0.113498, p-value = 0.0304; Novel)
- Trigenic negative genetic interactions in this triple mutant Synthetic genetic array (SGA) analysis were considered to be significant when tau < -0.08 and p < 0.05.
Curated By
- BioGRID