BAIT
SRN2
SRN10, VPL16, VPS37, ESCRT-I subunit protein SRN2, L000003103, YLR119W
Component of the ESCRT-I complex; ESCRT-I is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus
GO Process (3)
GO Function (1)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- ESCRT I complex [IDA, IPI]
- endosome [IPI]
Saccharomyces cerevisiae (S288c)
PREY
MCM5
BOB1, CDC46, MCM DNA helicase complex subunit MCM5, L000000279, YLR274W
Component of the Mcm2-7 hexameric helicase complex; MCM complex is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase
GO Process (8)
GO Function (5)
GO Component (7)
Gene Ontology Biological Process
- DNA replication initiation [IMP]
- chromatin silencing at telomere [IMP]
- double-strand break repair via break-induced replication [IMP]
- establishment of chromatin silencing [IMP]
- negative regulation of chromatin silencing at telomere [IMP]
- nuclear DNA replication [IMP]
- pre-replicative complex assembly involved in nuclear cell cycle DNA replication [IDA, IPI]
- regulation of DNA-dependent DNA replication initiation [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Negative Genetic
Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.
Publication
Systematic analysis of complex genetic interactions.
To systematically explore complex genetic interactions, we constructed ~200,000 yeast triple mutants and scored negative trigenic interactions. We selected double-mutant query genes across a broad spectrum of biological processes, spanning a range of quantitative features of the global digenic interaction network and tested for a genetic interaction with a third mutation. Trigenic interactions often occurred among functionally related genes, and ... [more]
Science Apr. 20, 2018; 360(6386); [Pubmed: 29674565]
Quantitative Score
- -0.287395 [Confidence Score]
Throughput
- High Throughput
Ontology Terms
- phenotype: colony size (APO:0000063)
Additional Notes
- Trigenic interaction: Query allele name: srn2-delta+rim21-delta; Array allele name: cdc46-1 (GI score = -0.287395, p-value = 0.00135; Modified_Q-)
- Trigenic negative genetic interactions in this triple mutant Synthetic genetic array (SGA) analysis were considered to be significant when tau < -0.08 and p < 0.05.
Curated By
- BioGRID